Braun, Christina M. and Hackert, Philipp and Schmid, Catharina E. and Bohnsack, Markus T. and Bohnsack, Katherine E. and Perez-Fernandez, Jorge (2020) Pol5 is required for recycling of small subunit biogenesis factors and for formation of the peptide exit tunnel of the large ribosomal subunit. NUCLEIC ACIDS RESEARCH, 48 (1). pp. 405-420. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
More than 200 assembly factors (AFs) are required for the production of ribosomes in yeast. The step-wise association and dissociation of these AFs with the pre-ribosomal subunits occurs in a hierarchical manner to ensure correct maturation of the prerRNAs and assembly of the ribosomal proteins. Although decades of research have provided a wealth of insights into the functions of many AFs, others remain poorly characterized. Pol5 was initially classified with B-type DNA polymerases, however, several lines of evidence indicate the involvement of this protein in ribosome assembly. Here, we show that depletion of Pol5 affects the processing of pre-rRNAs destined for the both the large and small subunits. Furthermore, we identify binding sites for Pol5 in the 5' external transcribed spacer and within domain III of the 25S rRNA sequence. Consistent with this, we reveal that Pol5 is required for recruitment of ribosomal proteins that form the polypeptide exit tunnel in the LSU and that depletion of Pol5 impairs the release of 5' ETS fragments from early pre-40S particles. The dual functions of Pol5 in 60S assembly and recycling of pre-40S AFs suggest that this factor could contribute to ensuring the stoichiometric production of ribosomal subunits.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SACCHAROMYCES-CEREVISIAE; PROCESSING STEPS; NUCLEAR EXOSOME; 90S PRERIBOSOME; PRE-RIBOSOME; RNA; YEAST; MATURATION; SITES; BINDING; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > House of the Ribosome |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Apr 2021 11:21 |
| Last Modified: | 01 Apr 2021 11:21 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45355 |
Actions (login required)
 |
View Item |
