Troebs, Johannes and Asam, Claudia and Pion, Eric and Prantl, Lukas and Aung, Thiha and Haerteis, Silke (2020) 3D monitoring of tumor volume in an in vivo model. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, 76 (2). pp. 123-131. ISSN 1386-0291, 1875-8622
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: The ability to evaluate tumor development within experimental oncology is of upmost importance. However, determining tumor volumes in 3D in vivo tumor models is challenging. The chick chorioallantoic membrane (CAM) model represents an optimized xenograft model that surpasses many disadvantages that are inherent to rodent models and provides the opportunity of real-time monitoring of tumor growth. OBJECTIVE: The objective of this study was to introduce a new method that enables monitoring of tumor growth within the CAM model throughout the course of the experiment. METHODS: Sarcoma cell lines and sarcoma primary tumors were grafted onto the CAM of fertilized chicken eggs. A digital microscope (Keyence VHX-6000) was used for 3D volume monitoring before and after tumor excision and compared it to tumor weight. RESULTS: Accuracy of tumor volumes was validated through correlation with tumor weight. In and ex ovo tumor volumes correlated significantly with tumor weight values. CONCLUSIONS: The described method can be used to assess the effects of chemotherapeutic agents on the growth of tumors that have been grafted onto the CAM and further advance personalized cancer therapy. In summary, we established a promising protocol that enables in vivo real-time tracking of tumor growth in the CAM model using a digital microscope.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHORIOALLANTOIC MEMBRANE; PROGNOSTIC-FACTORS; SOFT-TISSUE; RHABDOMYOSARCOMA; SARCOMA; CHILDHOOD; EMBRYO; Chorioallantoic membrane (CAM); in vivo tumor model; tumor profiling; rhabdomyosarcoma; sarcoma; volume measurement; 3D volume |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2021 08:46 |
| Last Modified: | 06 Apr 2021 08:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45397 |
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