Bakhtiar, Hengameh and Mazidi, Amir and Mohammadi-Asl, Saeed and Hasannia, Sadegh and Ellini, Mohammad Reza and Pezeshki-Modaress, Mohammad and Ostad, Seyed Naser and Galler, Kerstin and Azarpazhooh, Amir and Kishen, Anil (2020) Potential of Treated Dentin Matrix Xenograft for Dentin-Pulp Tissue Engineering. JOURNAL OF ENDODONTICS, 46 (1). pp. 57-64. ISSN 0099-2399, 1878-3554
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Introduction: This study aims to develop and characterize the regenerative potential of an atelopeptidized treated dentin matrix xenograft using in vitro and in vivo models. Methods: Freshly extracted bovine dentin was pulverized into 250- to 500-mu m particles and demineralized with 17% EDTA for 1, 7, and 13 days. The samples were atelopeptidized with pepsin. The degree of demineralization and the effect of atelopeptidization were assessed using field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy, respectively. The expression of dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, and osteopontin was evaluated in dental pulp stem cells using quantitative real-time polymerase chain reaction. The samples were then implanted intramuscularly in rats for 30 days, and the inflammatory cells were quantified histologically. Results: Field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy revealed an exposed tubular structure of dentin after 1 and 7 days of demineralization. Fourier transform infrared spectroscopy confirmed the absence of amide peaks at 1260 to 1640/cm after atelopeptidization. The dental pulp stem cell expression of dentin matrix acidic phosphoprotein 1 and dentin sialophosphoprotein increased in all compared with the untreated control group (P < .05). The maximum expression rates were observed for the 1-day demineralized and atelopeptidized group. The 1-day demineralized group elicited the highest inflammatory response compared with the 7- or 13-day demineralized groups (P < .001). Atelopeptidization significantly decreased the inflammatory response only in the 1-day demineralized dentin group (P < .05). Conclusions: Atelopeptidization of 1-day demineralized dentin xenograft preserved the collagen structure, minimized the immune reaction, and provided sufficient regenerative potential.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-FORMATION; COLLAGEN; SIZE; Atelopeptidization; demineralization; dentin-pulp complex; regeneration; treated dentin matrix; xenograft |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2021 09:24 |
| Last Modified: | 08 Apr 2021 09:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45544 |
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