Bakhtiar, Hengameh and Pezeshki-Modaress, Mohammad and Kiaipour, Zahra and Shafiee, Mahdieh and Ellini, Mohammad Reza and Mazidi, Amir and Rajabi, Sarah and Benisi, Soheila Zamanlui and Ostad, Seyed Naser and Galler, Kerstin and Pakshir, Pardis and Azarpazhooh, Amir and Kishen, Anil (2020) Pulp ECM-derived macroporous scaffolds for stimulation of dental-pulp regeneration process. DENTAL MATERIALS, 36 (1). pp. 76-87. ISSN 0109-5641, 1879-0097
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Objective. Recent studies suggest xenogeneic extracellular matrices as potential regenerative tools in dental pulp regeneration. This study aimed to fabricate and characterize a novel three-dimensional macroporous pulp-derived scaffold that enables the attachment, penetration, proliferation and differentiation of mesenchymal stem cells. Method. Bovine pulp was decellularized and characterized with histological and DNA content methods. This scaffold was prepared using finely milled lyophilized decellularized pulp extracellular matrix (ECM) digested with pepsin. Three different concentrations of ECM (1.50, 2.25 and 3.00 mg/ml) were freeze-dried and were tested with/without chemical crosslinking. The specimens were subjected to physicochemical characterization, cell viability and quantitative real time polymerase chain reaction assessments with human bone marrow mesenchymal stem cells (hBMMSCs). All scaffolds were subcutaneously implanted in rats for two weeks and histological and immunostaining analyses were performed. Results. Histological and DNA analysis confirmed complete decellularization. All samples demonstrated more than 97% porosity and 1.50 mg/ml scaffold demonstrated highest water absorption. The highest cell viability and proliferation of hBMMSCs was observed on the 3.00 mg/ml crosslinked scaffolds. The gene expression analysis showed a significant increase of dmp-1 and collagen-I on 3.00 mg/ml crosslinked scaffolds compared to the other scaffolds. Histological examination of subcutaneous implanted scaffolds revealed low immunological response, and enhanced angiogenesis in cross-linked samples compared to non-crosslinked samples. Significance. The three-dimensional macroporous pulp-derived injectable scaffold developed and characterized in this study displayed potential for regenerative therapy. While the scaffold biodegradability was decreased by crosslinking, the biocompatibility of post-crosslinked scaffold was significantly improved. (C) 2019 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; DIFFERENTIATION; TISSUE; SIZE; Tissue engineering; Dentin-pulp complex; Pulp ECM; Crosslinked scaffold |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Apr 2021 09:36 |
| Last Modified: | 08 Apr 2021 09:36 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45550 |
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