Eckl, Daniel Bernhard and Eben, Stefanie Susanne and Schottenhaml, Laura and Eichner, Anja and Vasold, Rudolf and Spaeth, Andreas and Baeumler, Wolfgang and Huber, Harald (2021) Interplay of phosphate and carbonate ions with flavin photosensitizers in photodynamic inactivation of bacteria. PLOS ONE, 16 (6): e0253212. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Photodynamic inactivation (PDI) of pathogenic bacteria is a promising technology in different applications. Thereby, a photosensitizer (PS) absorbs visible light and transfers the energy to oxygen yielding reactive oxygen species (ROS). The produced ROS are then capable of killing microorganisms via oxidative damage of cellular constituents. Among other PS, some flavins are capable of producing ROS and cationic flavins are already successfully applied in PDI. When PDI is used for example on tap water, PS like flavins will encounter various ions and other small organic molecules which might hamper the efficacy of PDI. Thus, the impact of carbonate and phosphate ions on PDI using two different cationic flavins (FLASH-02a, FLASH-06a) was investigated using Staphylococcus aureus and Pseudomonas aeruginosa as model organisms. Both were inactivated in vitro at a low light exposure of 0.72 J cm(-)(2). Upon irradiation, FLASH-02a reacts to single substances in the presence of carbonate or phosphate, whereas the photochemical reaction for FLASH-06a was more unspecific. DPBF-assays indicated that carbonate and phosphate ions decreased the generation of singlet oxygen of both flavins. Both microorganisms could be easily inactivated by at least one PS with up to 6 log(10) steps of cell counts in low ion concentrations. Using the constant radiation exposure of 0.72 J cm(-2), the inactivation efficacy decreased somewhat at medium ion concentrations but reached almost zero for high ion concentrations. Depending on the application of PDI, the presence of carbonate and phosphate ions is unavoidable. Only upon light irradiation such ions may attack the PS molecule and reduce the efficacy of PDI. Our results indicate concentrations for carbonate and phosphate, in which PDI can still lead to efficient reduction of bacterial cells when using flavin based PS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SINGLET OXYGEN; METHYLENE-BLUE; WASTE-WATER; FATTY-ACIDS; RIBOFLAVIN; PATHOGENS; THERAPY; PHOTOINACTIVATION; OXIDATION; CURCUMIN; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) Chemistry and Pharmacy > Institut für Organische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Jul 2022 06:20 |
| Last Modified: | 05 Jul 2022 06:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45579 |
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