Pfirschke, Christina and Gebhardt, Christoffer and Zoernig, Inka and Pritsch, Maria and Eichmueller, Stefan B. and Jaeger, Dirk and Enk, Alexander and Beckhove, Philipp (2015) T cell responses in early-stage melanoma patients occur frequently and are not associated with humoral response. CANCER IMMUNOLOGY IMMUNOTHERAPY, 64 (11). pp. 1369-1381. ISSN 0340-7004, 1432-0851
Full text not available from this repository. (Request a copy)Abstract
Endogenous tumor-specific T cells are detectable in patients with different tumor types including malignant melanoma (MM). They can control tumor growth, have impact on patient survival and correlate with improved clinical response to immune checkpoint therapy. Thus, they may represent a potent biomarker for respective treatment decisions. So far, major target antigens of endogenous MM-reactive T cells have not been determined systematically. Instead, autoantibodies are discussed as surrogate parameter for MM-specific T cells. Throughout a period of more than 60 days after tumor resection, we therefore determined in 38 non-metastasized primary MM patients and in healthy individuals by IFN gamma ELISpot and bead-based fluorescent multiplex assay major target antigens of spontaneous T cell and humoral responses using a broad panel of MM antigens and assessed the presence and suppressive impact of MM-reactive regulatory T cells (Tregs). We show that MM-reactive T cells are frequent in MM patients, transiently increase after tumor removal and are mostly directed against Melan-A/MART-1, Tyrosinase, NA17-A and p53. MM-specific Tregs were only detected in few patients and inhibited MM-reactive T cells particularly early after tumor resection. Tumor-specific autoantibodies occurred in most patients, but did not correlate with T cell responses. Thus, endogenous antibodies may not be reliable surrogate parameters of MM-reactive T cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BREAST-CANCER PATIENTS; TUMOR-INFILTRATING LYMPHOCYTES; BONE-MARROW; ANTIBODY-RESPONSES; COLORECTAL-CANCER; LUNG-CANCER; PROGNOSTIC-SIGNIFICANCE; METASTATIC MELANOMA; PANCREATIC-CANCER; PEPTIDE VACCINES; Malignant melanoma; Memory T cells; Autoantibodies; Regulatory T cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 May 2019 13:59 |
| Last Modified: | 07 May 2019 13:59 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4560 |
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