Kheiroddin, Parastoo and Schoeberl, Patricia and Althammer, Michael and Cibali, Ezgi and Wuerfel, Thea and Wein, Hannah and Kulawik, Birgit and Buntrock-Doepke, Heike and Weigl, Eva and Gran, Silvia and Gruendl, Magdalena and Langguth, Jana and Lampl, Benedikt and Judex, Guido and Niggel, Jakob and Pagel, Philipp and Schratzenstaller, Thomas and Schneider-Brachert, Wulf and Gastiger, Susanne and Bodenschatz, Mona and Konrad, Maike and Levchuk, Artem and Roth, Cornelius and Schoener, David and Schneebauer, Florian and Rohrmanstorfer, Rene and Dekens, Marcus P. and Brandstetter, Susanne and Zuber, Johannes and Wallerstorfer, Daniel and Burkovski, Andreas and Ambrosch, Andreas and Wagner, Thomas and Kabesch, Michael (2021) Results of WICOVIR Gargle Pool PCR Testing in German Schools Based on the First 100,000 Tests. FRONTIERS IN PEDIATRICS, 9: 721518. ISSN 2296-2360,
Full text not available from this repository. (Request a copy)Abstract
Background: Opening schools and keeping children safe from SARS-CoV-2 infections at the same time is urgently needed to protect children from direct and indirect consequences of the COVID-19 pandemic. To achieve this goal, a safe, efficient, and cost-effective SARS-CoV-2 testing system for schools in addition to standard hygiene measures is necessary.</p> Methods: We implemented the screening WICOVIR concept for schools in the southeast of Germany, which is based on gargling at home, pooling of samples in schools, and assessment of SARS-CoV-2 by pool rRT-PCR, performed decentralized in numerous participating laboratories. Depooling was performed if pools were positive, and results were transmitted with software specifically developed for the project within a day. Here, we report the results after the first 13 weeks in the project.</p> Findings: We developed and implemented the proof-of-concept test system within a pilot phase of 7 weeks based on almost 17,000 participants. After 6 weeks in the main phase of the project, we performed >100,000 tests in total, analyzed in 7,896 pools, identifying 19 cases in >100 participating schools. On average, positive children showed an individual CT value of 31 when identified in the pools. Up to 30 samples were pooled (mean 13) in general, based on school classes and attached school staff. All three participating laboratories detected positive samples reliably with their previously established rRT-PCR standard protocols. When self-administered antigen tests were performed concomitantly in positive cases, only one of these eight tests was positive, and when antigen tests performed after positive pool rRT-PCR results were already known were included, 3 out of 11 truly positive tests were also identified by antigen testing. After 3 weeks of repetitive WICOVIR testing twice weekly, the detection rate of positive children in that cohort decreased significantly from 0.042 to 0.012 (p = 0.008).</p> Interpretation: Repeated gargle pool rRT-PCR testing can be implemented quickly in schools. It is an effective, valid, and well-received test system for schools, superior to antigen tests in sensitivity, acceptance, and costs.</p>
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COVID-19; CHILDREN; children; COVID-19; Germany; PCR; pooling; gargle; schools; pandemic |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Medicine > Lehrstuhl für Kinder- und Jugendmedizin Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Jul 2022 09:40 |
| Last Modified: | 19 Jul 2022 09:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/45831 |
Actions (login required)
 |
View Item |
