Koellen, Karin and Dietz, Lena and Bies-Etheye, Natacha and Lagrange, Thierry and Grasser, Marion and Grasser, Klaus D. (2015) The zinc-finger protein SPT4 interacts with SPT5L/KTF1 and modulates transcriptional silencing in Arabidopsis. FEBS LETTERS, 589 (21). pp. 3254-3257. ISSN 0014-5793, 1873-3468
Full text not available from this repository. (Request a copy)Abstract
The Arabidopsis multidomain protein SPT5L/KTF1 (which has similarity to the transcript elongation factor SPT5) associates with RNA polymerase V (RNAPV) and is an accessory factor in RNA-directed DNA methylation. The zinc-finger protein SPT4 was found to interact with SPT5L (and SPT5) both in vivo and in vitro. Here, we show that plants depleted of SPT4 relative to wild type display reduced DNA methylation and the locus specificity is shared with SPT5L, suggesting a cooperation of SPT4 and SPT5L. Unlike observed for SPT5, no reduced protein level of SPT5L is determined in SPT4-deficient plants. These experiments demonstrate that in addition to the RNA polymerase II-associated SPT4/SPT5 that is generally conserved in eukaryotes, flowering plants have SPT4/SPT5L that is involved in RNAPV-mediated transcriptional silencing. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DIRECTED DNA METHYLATION; RNA-POLYMERASE-II; SPT4-SPT5 COMPLEX; NONCODING RNA; ELONGATION; IV; SPT4/SPT5; CHROMATIN; SUBUNITS; REQUIRES; SPT4/SPT5L; Chromatin; Gene expression; RNA-directed DNA methylation (RdDM); RNA polymerase V |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Jun 2019 13:13 |
| Last Modified: | 04 Jun 2019 13:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4598 |
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