Wolfbeis, Otto S. (2021) Fluorescent chameleon labels for bioconjugation and imaging of proteins, nucleic acids, biogenic amines and surface amino groups. a review. METHODS AND APPLICATIONS IN FLUORESCENCE, 9 (4): 042001. ISSN 2050-6120
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Chameleon labels (ChLs) possess the unique property of changing (visible) color and fluorescence on binding to amino groups of biomolecules. MostChLs react with primary aliphatic amino groups such as those in lysine or with amino groups artificially introduced into polynucleic acids or saccharides, but someothers also react with secondary amino groups. Under controlled circumstances, the reactions are fairly specific. The review is subdivided into the following sections: (1) An introduction and classification of fluorescent labels; (2) pyrylium labels that undergo shortwave color changes upon labelling, typically from blue to red; (3) polymethine type of labels (that also undergo shortwave color changes, typically from green to blue; (4) various other (less common) chromogenic and fluorogenic systems; (5) hemicyanine labels that undergo longwave color changes, typically from yellow to purple; (6) the application of ChLs to labeling of proteins and oligonucleotides; (7) applications to fluorometric assays and sensing; (8) applications to fluorescence imaging of biomolecules; (9) applications in studies on affinity interactions (receptor-ligand binding); (10) applications in surface and interface chemistry; and (11) applications in chromatography, electrophoresis and isotachophoresis of biomolecules.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SDS-GEL ELECTROPHORESIS; FLUOROGENIC REAGENTS; CAPILLARY-ELECTROPHORESIS; MICROTITER PLATE; MASS DETECTION; DYES; PH; SEPARATION; ALBUMIN; MICROALBUMINURIA; labels; bioconjugation; protein labelling; imaging; fluorometric assays; electrophoresis; biogenic amines |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Petra Gürster |
| Date Deposited: | 16 Dec 2022 12:00 |
| Last Modified: | 16 Dec 2022 12:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46029 |
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