Haehnel, Viola and Brosig, Andreas-Michael and Ehrenschwender, Martin and Burkhardt, Ralph and Offner, Robert and Ahrens, Norbert (2021) Apoptosis induction by extracorporeal photopheresis is enhanced by increasing the 8-methoxypsoralen concentration and by replacing plasma with saline. TRANSFUSION, 61 (10). pp. 2991-2999. ISSN 0041-1132, 1537-2995
Full text not available from this repository. (Request a copy)Abstract
Background Extracorporeal photopheresis (ECP), an apheresis-based therapy for various immunological diseases, works mainly by inducing apoptosis in lymphocytes. Several factors influence the efficacy of ECP with the photosensitizer 8-methoxypsoralen (8-MOP) and ultraviolet light A (UVA). This study aimed to optimize treatment by varying the 8-MOP starting concentration and the cell suspension medium. Materials and Methods All patients (n = 13) included in this study received photopheresis as medically indicated. Cells collected with a Spectra Optia apheresis system were suspended in plasma or physiological saline (NaCl) and incubated with 200 ng/ml versus 340 ng/ml photosensitizer before UVA irradiation (Macogenic G2 or UVA PIT system). Lymphocyte apoptosis and caspase activity were analyzed by flow cytometry and fluorimetry, and residual 8-methoxypsoralen concentrations by liquid chromatography-mass spectrometry. Results Raising the 8-MOP starting concentration significantly increased lymphocyte apoptosis, with values of 22% versus 35% (plasma) and 28%-46% (NaCl) at 24 h post-ECP and 37% versus 86% (plasma) and 74% versus 97% (NaCl) at 48 h for 200 ng/ml versus 340 ng/ml. Pre-transfusion residual 8-MOP levels were 168 ng/ml (plasma) and 162 ng/ml (NaCl) versus 290 ng/ml (plasma) and 266 ng/ml (NaCl) for the lower versus higher dose, respectively. Discussion Hence, 8-MOP concentration influences the efficacy of photopheresis as lymphocyte apoptosis rates were significantly higher with the higher starting concentration and with NaCl versus plasma. This indicates that increased 8-MOP starting doses and saline as additional suspension medium could help in improving ECP's efficacy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DENDRITIC CELLS; IN-VITRO; THERAPEUTIC APHERESIS; WRITING COMMITTEE; CLINICAL-PRACTICE; AMERICAN SOCIETY; QUALITY-CONTROL; T-CELLS; PHOTOCHEMOTHERAPY; PHOTOCHEMISTRY; 8-methoxypsoralen; apoptosis; ECP; extracorporeal photopheresis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Aug 2022 12:37 |
| Last Modified: | 02 Aug 2022 12:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46039 |
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