Schmid, Maximilian and Dufner, Bianca and Duerk, Julius and Bedal, Konstanze and Stricker, Kristina and Prokoph, Lukas Ali and Koch, Christoph and Wege, Anja K. and Zirpel, Henner and van Zandbergen, Ger and Ecker, Rupert and Boghiu, Bogdan and Ritter, Uwe (2015) An Emerging Approach for Parallel Quantification of Intracellular Protozoan Parasites and Host Cell Characterization Using TissueFAXS Cytometry. PLOS ONE, 10 (10): e0139866. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Characterization of host-pathogen interactions is a fundamental approach in microbiological and immunological oriented disciplines. It is commonly accepted that host cells start to change their phenotype after engulfing pathogens. Techniques such as real time PCR or ELISA were used to characterize the genes encoding proteins that are associated either with pathogen elimination or immune escape mechanisms. Most of such studies were performed in vitro using primary host cells or cell lines. Consequently, the data generated with such approaches reflect the global RNA expression or protein amount recovered from all cells in culture. This is justified when all host cells harbor an equal amount of pathogens under experimental conditions. However, the uptake of pathogens by phagocytic cells is not synchronized. Consequently, there are host cells incorporating different amounts of pathogens that might result in distinct pathogen-induced protein biosynthesis. Therefore, we established a technique able to detect and quantify the number of pathogens in the corresponding host cells using immunofluorescence-based high throughput analysis. Paired with multicolor staining of molecules of interest it is now possible to analyze the infection profile of host cell populations and the corresponding phenotype of the host cells as a result of parasite load.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EXPERIMENTAL CUTANEOUS LEISHMANIASIS; REAL-TIME PCR; DENDRITIC CELLS; BALB/C MICE; IN-VIVO; MACROPHAGES; MICROSCOPY; GAMMA; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 May 2019 11:55 |
| Last Modified: | 13 May 2019 11:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4604 |
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