Kupke, Laura S. and Arndt, Stephanie and Lenzer, Simon and Metz, Sophia and Unger, Petra and Zimmermann, Julia L. and Bosserhoff, Anja-Katrin and Gruber, Michael and Karrer, Sigrid (2021) Cold Atmospheric Plasma Promotes the Immunoreactivity of Granulocytes In Vitro. BIOMOLECULES, 11 (6): 902. ISSN , 2218-273X
Full text not available from this repository. (Request a copy)Abstract
Cold atmospheric plasma (CAP) reduces bacteria and interacts with tissues and cells, thus improving wound healing. The CAP-related induction of neutrophils was recently described in stained sections of wound tissue in mice. Consequently, this study aimed to examine the functionality of human polymorphonuclear cells (PMN)/granulocytes through either a plasma-treated solution (PTS) or the direct CAP treatment with different plasma modes and treatment durations. PTS analysis yielded mode-dependent differences in the production of reactive oxygen species (ROS) and reactive nitrogen species (RNS) after CAP treatment. Live-cell imaging did not show any chemo-attractive or NETosis-inducing effect on PMNs treated with PTS. The time to maximum ROS production (TmaxROS) in PMNs was reduced by PTS and direct CAP treatment. PMNs directly treated with CAP showed an altered cell migration dependent on the treatment duration as well as decreased TmaxROS without inducing apoptosis. Additionally, flow cytometry showed enhanced integrin and selectin expression, as a marker of activation, on PMN surfaces. In conclusion, the modification of PMN immunoreactivity may be a main supporting mechanism for CAP-induced improvement in wound healing.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRESSURE PLASMA; L-SELECTIN; KERATINOCYTES; NEUTROPHILS; EXPRESSION; MIGRATION; ADHESION; NETOSIS; IMPACT; WOUNDS; cold atmospheric plasma; wound treatment; granulocytes; live-cell imaging; NETosis; flow cytometry |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Anästhesiologie Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Aug 2022 08:55 |
| Last Modified: | 09 Aug 2022 08:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46204 |
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