Ahmad, Misbha Ud Din and Waege, Ingrid and Hausner, Winfried and Thomm, Michael and Boos, Winfried and Diederichs, Kay and Welte, Wolfram (2015) Structural Insights into Nonspecific Binding of DNA by TrmBL2, an Archaeal Chromatin Protein. JOURNAL OF MOLECULAR BIOLOGY, 427 (20). pp. 3216-3229. ISSN 0022-2836, 1089-8638
Full text not available from this repository. (Request a copy)Abstract
The crystal structure of TrmBL2 from the archaeon Pyrococcus furiosus shows an association of two pseudosymmetric dimers. The dimers follow the prototypical design of known bacterial repressors with two helix turn helix (HTH) domains binding to successive major grooves of the DNA. However, in TrmBL2, the two dimers are arranged at a mutual displacement of approximately 2 bp so that they associate with the DNA along the double-helical axis at an angle of approximately 80 degrees. While the deoxyribose phosphate groups of the double-stranded DNA (dsDNA) used for co-crystallization are clearly seen in the electron density map, most of the nucleobases are averaged out. Refinement required to assume a superposition of at least three mutually displaced dsDNAs. The HTH domains interact primarily with the deoxyribose phosphate groups and polar interactions with the nucleobases are almost absent. This hitherto unseen mode of DNA binding by TrmBL2 seems to arise from nonoptimal protein-DNA contacts made by its four HTH domains resulting in a low-affinity, nonspecific binding to DNA. (c) 2015 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLOBAL TRANSCRIPTIONAL REGULATOR; CRYSTAL-STRUCTURE; WINGED-HELIX; PYROCOCCUS-FURIOSUS; THERMOCOCCUS-KODAKARENSIS; PHOSPHOLIPID SYNTHASES; SECONDARY-STRUCTURE; ABC TRANSPORTER; H-NS; COMPLEX; TrmBL2; nonspecific DNA binding; chromatin protein; protein-DNA complex; Archaea |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 May 2019 12:53 |
| Last Modified: | 13 May 2019 12:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4625 |
Actions (login required)
 |
View Item |
