Beck, Franziska and Horn, Carina and Baeumner, Antje J. (2022) Dry-reagent microfluidic biosensor for simple detection of NT-proBNP via Ag nanoparticles. ANALYTICA CHIMICA ACTA, 1191: 339375. ISSN 0003-2670, 1873-4324
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The diagnosis of many diseases requires monitoring of biomarker levels over a period of time instead of assessing their concentration only once. For example, in case of heart failure determination, the levels of N-terminal prohormone brain natriuretic peptide (NT-proBNP) in blood vary so strongly amongst individuals, that the current procedure of one-time measurement in combination with clinical examination does not allow for accurate assessment of disease severity and progression. Our microfluidic biosensor addresses key characteristics of desirable home-tests which include low limits of detection, small sample volume (less than 10 mL), simple detection strategies, and ready-to-go all-dried long-term stable reagents. Here, electrochemically superior silver nanoparticles (AgNP) were dried directly within the microfluidic channel in a matrix of trehalose sugar doped with Na2SO3 as oxygen scavenger. This successfully prevented AgNP oxidation and enabled dry and ready-to-use storage for at least 18 weeks. Based on this, laser-cut flow chips were developed containing all bioassay reagents needed in a ready-togo dry format. An oxidation-reduction stripping voltammetry strategy was used for highly sensitive quantification of the AgNPs as electrochemical label. This microfluidic biosensor demonstrated limits of detection for NT-proBNP of 0.57 ng mL-1 with a mean error of 6% (n > 3) in undiluted human serum, which is below the clinically relevant cut-off of 1 ng mL-1. This practical approach has the potential to substitute commonly used lateral-flow assays for various biomarkers, as it offers low patient sample volumes hence supporting simple finger-prick strategies well-known also for other electrochemical biosensors, and independence from the notorious variability in fleece fabrication. (c) 2021 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTROCHEMICAL DETECTION; HEART-FAILURE; SILVER; PAPER; Electrochemical biosensor; Silver nanoparticle; Microfluidic; Blood analysis; Differential pulse voltammetry |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Aug 2022 08:52 |
| Last Modified: | 10 Aug 2022 08:52 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46279 |
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