Kikhney, J. and von Schoening, D. and Steding, I. and Schulze, J. and Petrich, A. and Hiergeist, A. and Reischl, U. and Moter, A. and Thomas, A. (2017) Is Ureaplasma spp. the leading causative agent of acute chorioamnionitis in women with preterm birth? CLINICAL MICROBIOLOGY AND INFECTION, 23 (2): e7. ISSN 1198-743X, 1469-0691
Full text not available from this repository. (Request a copy)Abstract
Objectives: Aim of this study was to detect microorganisms in fetal membranes and placental tissue in preterm chorioamnionitis by combining fluorescence in situ hybridization (FISH) with broad range PCR. The combination of the two molecular techniques enables identification and localization of the microorganisms within the tissue, confirming their clinical relevance. Methods: In a prospective cohort study, we compared 31 women with preterm premature rupture of membranes or preterm labour and preterm delivery by caesarean section with a control group of 26 women undergoing elective caesarean section at term. Fetal membranes and placental tissue were analysed by FISH and broad range 16S rRNA- gene PCR and sequencing. Results: For 20 women in the preterm group, caesarean section was performed because of a clinical diagnosis of chorioamnionitis. Microorganisms were detected in the tissues by both molecular techniques in 11 out of 20 women. Among those, Ureaplasma spp. was most abundant, with five cases that remained culture-negative and would have been missed by routine diagnostic procedures. Other infections were caused by Staphylococcus aureus, Streptococcus mitis or Escherichia coli. FISH and PCR were negative for all women without suspected chorioamnionitis and for the control group. Conclusions: Combination of FISH with broad- range PCR and sequencing permitted unambiguous identification of the causative microorganisms in chorioamnionitis. The high prevalence of Ureaplasma spp. should lead to a re-evaluation of its clinical significance and possible therapeutic consequences. (C) 2016 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-SITU HYBRIDIZATION; TARGETED OLIGONUCLEOTIDE PROBES; FETAL MEMBRANES; ETIOLOGIC AGENTS; AMNIOTIC-FLUID; MICROORGANISMS; DIVERSITY; LABOR; IDENTIFICATION; ENDOCARDITIS; 16S rRNA; Chorioamnionitis; Fluorescence in situ hybridization; Neonatal mortality; Preterm birth; Ureaplasma |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:01 |
| Last Modified: | 14 Feb 2019 11:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/463 |
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