Salehi, Mohammad Saied and Neumann, Inga D. and Jurek, Benjamin and Pandamooz, Sareh (2021) Co-Stimulation of Oxytocin and Arginine-Vasopressin Receptors Affect Hypothalamic Neurospheroid Size. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22 (16): 8464. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Oxytocin (OXT) is a neuropeptide involved in a plethora of behavioral and physiological processes. However, there is a prominent lack of 3D cell culture models that investigate the effects of OXT on a cellular/molecular level. In this study, we established a hypothalamic neuronal spheroid model to investigate the cellular response in a more realistic 3D setting. Our data indicate that the formation of spheroids itself does not alter the basic characteristics of the cell line and that markers of cellular morphology and connectivity are stably expressed. We found that both OXT and arginine vasopressin (AVP) treatment increase spheroid size (surface area and volume), as well as individual nucleus size, which serves as an indicator for cellular proliferation. The cellular response to both OXT and AVP seems mainly to be mediated by the AVP receptor 1a (V1aR); however, the OXT receptor (OXTR) contributes significantly to the observed proliferative effect. When we blocked the OXTR pharmacologically or knocked down the OXTR by siRNA, the OXT- or AVP-induced cellular proliferation decreased. In summary, we established a 3D cell culture model of the neuronal response to OXT and AVP and found that spheroids react to the treatment via their respective receptors but also via cross-talk between the two receptor types.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-LINE; NEURONS; ACTIVATION; EXPRESSION; UPSTREAM; BEHAVIOR; DISEASE; CULTURE; ANXIETY; oxytocin; spheroid; proliferation; OXTR; V1aR; cytoskeleton |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Aug 2022 12:07 |
| Last Modified: | 25 Aug 2022 12:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46383 |
Actions (login required)
 |
View Item |
