Prantl, Lukas and Eigenberger, Andreas and Brix, Eva and Kempa, Sally and Baringer, Magnus and Felthaus, Oliver (2021) Adipose Tissue-Derived Stem Cell Yield Depends on Isolation Protocol and Cell Counting Method. CELLS, 10 (5): 1113. ISSN , 2073-4409
Full text not available from this repository. (Request a copy)Abstract
In plastic surgery, lipofilling is a frequent procedure. Unsatisfactory vascularization and impaired cell vitality can lead to unpredictable take rates in the fat graft. The proliferation and neovascularization inducing properties of adipose tissue-derived stem cells may contribute to solve this problem. Therefore, the enrichment of fat grafts with stem cells is studied intensively. However, it is difficult to compare these studies because many factors-often not precisely described-are influencing the results. Our study summarizes some factors which influence the cell yield like harvesting, isolation procedure and quantification. Stem cells were isolated after liposuction. Quantification was done using a cell chamber, colony counting, or flow cytometry with changes to one parameter, only, for each comparison. Quantification of cells isolated after liposuction at the same harvesting site from the same patient can vary greatly depending on the details of the isolation protocol and the method of quantification. Cell yield can be influenced strongly by many factors. Therefore, a comparison of different studies should be handled with care.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STROMAL CELLS; IN-VITRO; INTERNATIONAL-SOCIETY; VASCULAR FRACTION; BONE-MARROW; FAT GRAFTS; DIFFERENTIATION; LIPOASPIRATE; VIABILITY; THERAPY; plastic surgery; adipose tissue; lipoaspirate; stem cells; fat grafting; cell yield; adipocyte viability |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Aug 2022 08:26 |
| Last Modified: | 29 Aug 2022 08:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46424 |
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