Reuschel, Edith and Toelge, Martina and Haeusler, Sebastian and Deml, Ludwig and Seelbach-Goebel, Birgit and Solano, Maria Emilia (2021) Perinatal Gram-Positive Bacteria Exposure Elicits Distinct Cytokine Responses In Vitro. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22 (1): 332. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
During pregnancy, infections caused by the gram-positive bacteria Enterococcus faecalis (E. faecalis), Streptococcus agalacticae (S. agalacticae), and Staphylococcus aureus (S. aureus) are major reasons for preterm labor, neonatal prematurity, meningitis, or sepsis. Here, we propose cytokine responses to bacterial infections by the immature perinatal immune system as central players in the pathogenesis of preterm birth and neonatal sepsis. We aimed to close the gap in knowledge about such cytokine responses by stimulating freshly isolated umbilical blood mononuclear cells (UBMC) with lysates of E. faecalis, S. agalacticae, and S. aureus collected from pregnant women in preterm labor. Bacterial lysates and, principally, S. aureus and S. agalacticae distinctly triggered most of the eleven inflammatory, anti-inflammatory, TH1/TH2 cytokines, and chemokines quantified in UBMC culture media. Chemokines depicted the most robust induction. Among them, MIP-1 beta was further enhanced in UBMC from female compered to male newborn infants. Due to its stability and high levels, we investigated the diagnostic value of IL-8. IL-8 was critically upregulated in cord blood of preterm neonates suffering from infections compared to gestational age-matched controls. Our results provide novel clues about perinatal immunity, underscoring a potential value of IL-8 for the timely detection of infections and suggesting that MIP-1 beta constitutes an early determinant of sex-specific immunity, which may contribute, e.g., to male's vulnerability to preterm birth.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEONATAL SEPSIS; STAPHYLOCOCCUS-AUREUS; INTRAUTERINE INFECTION; PRETERM BIRTH; PROTEIN-A; ENTEROCOCCUS; CELLS; SEX; INFLAMMATION; RELEASE; preterm birth; gram-positive bacterial infection; neonatal immunity; sex-specific immunity |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Aug 2022 08:32 |
| Last Modified: | 29 Aug 2022 08:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46430 |
Actions (login required)
 |
View Item |
