Kohl, Cynthia and Aung, Thiha and Haerteis, Silke and Papathemelis, Thomas (2021) Assessment of breast cancer primary tumor material in a 3D in vivo model. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, 79 (1). pp. 157-166. ISSN 1386-0291, 1875-8622
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: Breast cancer is the most common malignant tumor in women and highly heterogeneous with a variety of different molecular subtypes. The analysis of the individual tumor biology is necessary to develop a specific and individualized treatment plan for every patient. The chick chorioallantoic membrane (CAM) model, a 3D-in-vivo-tumor-model, could potentially provide a methodology that facilitates the gain of additional information regarding the tumor biology as well as the testing of the tumor's individual sensitivity to different therapies. OBJECTIVE: The objective was to establish the grafting of different breast cancer primaries onto the CAM for tumor profiling and the investigation of different parameters. METHODS: Breast cancer primary tissue of different patients was grafted onto the CAM. Subsequently, 3D volume and perfusion measurements were performed during the engraftment period. Histological analyses of the tumors were carried out after the engraftment period. RESULTS: The grafting of the breast cancer primaries onto the CAM was successful. The tumors remained partially vital and displayed angiogenic development on the CAM. CONCLUSIONS: Breast cancer primary material can be grafted onto the CAM and we observed visible and measurable changes of perfusion over time.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | EMBRYO CHORIOALLANTOIC MEMBRANE; ANGIOGENESIS; TISSUES; CAM; Breast cancer primary material; breast cancer; CAM model; 3D-in-vivo-tumor-model |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Aug 2022 12:41 |
| Last Modified: | 29 Aug 2022 12:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46470 |
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