Rink, Simone and Duerkop, Axel and Jacobi von Wangelin, Axel and Seidel, Michael and Baeumner, Antje J. (2021) Next generation luminol derivative as powerful benchmark probe for chemiluminescence assays. ANALYTICA CHIMICA ACTA, 1188: 339161. ISSN 0003-2670, 1873-4324
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Chemiluminescence (CL) provides outstanding analytical performance due to its independence from external light sources, background-free nature and exceptional sensitivity and selectivity. Yet, ultra-sensitive (bio)analysis is impeded by low hydrophilicity, poor quantum yields, fast kinetics or instability of most CL reagents such as luminol, acridinium esters, dioxetanes or peroxyoxalic derivatives. Photophysical studies show that m-carboxy luminol overcomes these limitations as its hydrophilic design provides a 5-fold increase in relative quantum yield resulting in superior performance in H2O2-dependent bioassays with 18-fold higher sensitivity for the quantification of its co-reactant H2O2, and 5-times lower detection limits for the luminophore. Studies with CL enhancers suggest its significance for mechanistic investigations in tandem with peroxidases. Finally, its integration into enzymatic and immunoassay applications demonstrates that m-carboxy luminol will provide signal enhancement, lower detection limits, and increased dynamic ranges for any other luminol-based CL assay, thus comprising the potential to replace luminol as benchmark probe. (C) 2021 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYDROGEN-PEROXIDE; BIOSENSORS; LACTATE; Analytical methods; Luminescence; Enzymatic lactate detection; Immunoassay; Luminol derivative |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 10 Aug 2022 11:47 |
| Last Modified: | 10 Aug 2022 11:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46484 |
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