Damrau, Christine and Colomb, Julien and Brembs, Bjoern (2021) Sensitivity to expression levels underlies differential dominance of a putative null allele of the Drosophila t ss h gene in behavioral phenotypes. PLOS BIOLOGY, 19 (5): e3001228. ISSN 1544-9173, 1545-7885
Full text not available from this repository. (Request a copy)Abstract
The biogenic amine octopamine (OA) and its precursor tyramine (TA) are involved in controlling a plethora of different physiological and behavioral processes. The tyramine-ss -hydroxylase (t ss h) gene encodes the enzyme catalyzing the last synthesis step from TA to OA. Here, we report differential dominance (from recessive to overdominant) of the putative null t ss h(nM18) allele in 2 behavioral measures in Buridan's paradigm (walking speed and stripe deviation) and in proboscis extension (sugar sensitivity) in the fruit fly Drosophila melanogaster. The behavioral analysis of transgenic t ss h expression experiments in mutant and wild-type flies as well as of OA and TA receptor mutants revealed a complex interaction of both aminergic systems. Our analysis suggests that the different neuronal networks responsible for the 3 phenotypes show differential sensitivity to t ss h gene expression levels. The evidence suggests that this sensitivity is brought about by a TA/OA opponent system modulating the involved neuronal circuits. This conclusion has important implications for standard transgenic techniques commonly used in functional genetics.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DOSAGE COMPENSATION; OCTOPAMINE RECEPTOR; TRANSGENIC RESCUE; TYRAMINE; NEURONS; INSECT; FLY; MODULATION; LOCOMOTION; DOPAMINE; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Neurogenetik (Prof. Dr. Björn Brembs) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Sep 2022 05:37 |
| Last Modified: | 05 Sep 2022 05:37 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46502 |
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