Forschbach, V. and Goppelt-Struebe, M. and Kunzelmann, K. and Schreiber, R. and Piedagnel, R. and Kraus, A. and Eckardt, K-U and Buchholz, B. (2015) Anoctamin 6 is localized in the primary cilium of renal tubular cells and is involved in apoptosis-dependent cyst lumen formation. CELL DEATH & DISEASE, 6: e1899. ISSN 2041-4889,
Full text not available from this repository. (Request a copy)Abstract
Primary cilia are antenna-like structures projected from the apical surface of various mammalian cells including renal tubular cells. Functional or structural defects of the cilium lead to systemic disorders comprising polycystic kidneys as a key feature. Here we show that anoctamin 6 (ANO6), a member of the anoctamin chloride channel family, is localized in the primary cilium of renal epithelial cells in vitro and in vivo. ANO6 was not essential for cilia formation and had no effect on in vitro cyst expansion. However, knockdown of ANO6 impaired cyst lumen formation of MDCK cells in three-dimensional culture. In the absence of ANO6, apoptosis was reduced and epithelial cells were incompletely removed from the center of cell aggregates, which form in the early phase of cystogenesis. In line with these data, we show that ANO6 is highly expressed in apoptotic cyst epithelial cells of human polycystic kidneys. These data identify ANO6 as a cilium-associated protein and suggest its functional relevance in cyst formation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED CHLORIDE SECRETION; CA2+-ACTIVATED CL-CHANNEL; CALCIUM; HYPOXIA; PROLIFERATION; MORPHOGENESIS; COMPONENT; PATHWAYS; TMEM16F; BNIP3; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie > Prof. Dr. Karl Kunzelmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Jun 2019 07:28 |
| Last Modified: | 06 Jun 2019 07:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4658 |
Actions (login required)
 |
View Item |
