Hoering, Marcus and Ejsing, Christer S. and Krautbauer, Sabrina and Ertl, Verena M. and Burkhardt, Ralph and Liebisch, Gerhard (2021) Accurate quantification of lipid species affected by isobaric overlap in Fourier-transform mass spectrometry. JOURNAL OF LIPID RESEARCH, 62: 100050. ISSN 0022-2275, 1539-7262
Full text not available from this repository. (Request a copy)Abstract
Lipidomics data require consideration of ions with near-identical masses, which comprises among others the Type-II isotopic overlap. This overlap occurs in series of lipid species differing only by number of double bonds (DBs) mainly because of the natural abundance of C-13-atoms. High-resolution mass spectrometry, such as Fourier-transform mass spectrometry (FTMS), is capable of resolving Type-II overlap depending on mass resolving power. In this work, we evaluated FTMS quantification accuracy of lipid species affected by Type-II overlap. Spike experiments with lipid species pairs of various lipid classes were analyzed by flow injection analysis-FTMS. Accuracy of quantification was evaluated without and with Type-II correction (using relative isotope abundance) as well as utilizing the first isotopic peak (M+1). Isobaric peaks, which were sufficiently resolved, were most accurate without Type-II correction. In cases of partially resolved peaks, we observed peak interference causing distortions in mass and intensity, which is a well-described phenomenon in FTMS. Concentrations of respective species were more accurate when calculated from M+1. Moreover, some minor species, affected by considerable Type-II overlap, could only be quantified by M+1. Unexpectedly, even completely unresolved peaks were substantially overcorrected by Type-II correction because of peak interference. The described method was validated including intraday and interday precisions for human serum and fibroblast samples. Taken together, our results show that accurate quantification of lipid species by FTMS requires resolution-depended data analysis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SHOTGUN LIPIDOMICS; EXTRACTS; TOOLS; lipidomics; mass spectrometry; lipids; phospholipids; sphingolipids; triglycerides; isotope correction; data processing; Fourier-transform mass spectrometry; peak interference |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 05 Sep 2022 08:27 |
| Last Modified: | 05 Sep 2022 08:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46603 |
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