Yagati, Ajay Kumar and Behrent, Arne and Tomanek, Vanessa and Chavan, Sachin Ganpat and Go, Anna and Park, Sung Ryul and Jin, Zhengzhi and Baeumner, Antje J. and Lee, Min-Ho (2022) Polypyrrole-palladium nanocomposite as a high-efficiency transducer for thrombin detection with liposomes as a label. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 414 (10). pp. 3205-3217. ISSN 1618-2642, 1618-2650
Full text not available from this repository. (Request a copy)Abstract
Sensitive and selective determination of protein biomarkers with high accuracy often remains a great challenge due to their existence in the human body at an exceptionally low concentration level. Therefore, sensing mechanisms that are easy to use, simple, and capable of accurate quantification of analyte are still in development to detect biomarkers at a low concentration level. To meet this end, we demonstrated a methodology to detect thrombin in serum at low concentration levels using polypyrrole (PPy)-palladium (Pd)nanoparticle-based hybrid transducers using liposomes encapsulated redox marker as a label. The morphology of Ppy-Pd composites was characterized by scanning electron microscopy, and the hybrid structure provided excellent binding and detection platform for thrombin detection in both buffer and serum solutions. For quantitative measurement of thrombin in PBS and serum, the change in current was monitored using differential pulse voltammetry, and the calculated limit of quantification (LOQ) and limit of detection (LOD) for the linear segment (0.1-1000 nM of thrombin) were 1.1 pM and 0.3 pM, in serum, respectively. The sensors also exhibited good stability and excellent selectivity towards the detection of thrombin, and thus make it a strong candidate for adopting its sensing applications in biomarker detection technologies.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REDUCED GRAPHENE OXIDE; MULTIWALLED CARBON NANOTUBES; PENCIL GRAPHITE ELECTRODE; ELECTROCHEMICAL FUNCTIONALIZATION; PD-POLYPYRROLE; BIOSENSOR; COMPOSITE; IMMUNOSENSOR; GENERATION; SENSOR; Polypyrrole; Palladium; Aptamer; Thrombin; Liposomes; Sensor; POC testing |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 18 Aug 2022 05:24 |
| Last Modified: | 18 Aug 2022 05:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46695 |
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