Arndt, Stephanie and Unger, Petra and Bosserhoff, Anja-Katrin and Berneburg, Mark and Karrer, Sigrid (2021) The Anti-Fibrotic Effect of Cold Atmospheric Plasma on Localized Scleroderma In Vitro and In Vivo. BIOMEDICINES, 9 (11): 1545. ISSN , 2227-9059
Full text not available from this repository. (Request a copy)Abstract
Cold Atmospheric Plasma (CAP) has shown promising results in the treatment of various skin diseases. The therapeutic effect of CAP on localized scleroderma (LS), however, has not yet been evaluated. We investigated the effects of CAP on LS by comparing human normal fibroblasts (hNF), human TGF-beta-activated fibroblasts (hAF), and human localized scleroderma-derived fibroblasts (hLSF) after direct CAP treatment, co-cultured with plasma-treated human epidermal keratinocytes (hEK) and with an experimental murine model of scleroderma. In hAF and hLSF, 2 min CAP treatment with the MicroPlaSter beta(R) plasma torch did not affect pro-fibrotic gene expression of alpha smooth muscle actin, fibroblast activating protein, and collagen type I, however, it promoted re-expression of matrix metalloproteinase 1. Functionally, CAP treatment reduced cell migration and stress fiber formation in hAF and hLSF. The relevance of CAP treatment was confirmed in an in vivo model of bleomycin-induced dermal fibrosis. In this model, CAP-treated mice showed significantly reduced dermal thickness and collagen deposition as well as a decrease in both alpha smooth muscle actin-positive myofibroblasts and CD68-positive macrophages in the affected skin in comparison to untreated fibrotic tissue. In conclusion, this study provides the first evidence for the successful use of CAP for treating LS and may be the basis for clinical trials including patients with LS.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BLEOMYCIN-INDUCED SCLERODERMA; HUMAN DERMAL FIBROBLASTS; SMOOTH MUSCLE ACTIN; ARGON PLASMA; CHRONIC WOUNDS; GENE-EXPRESSION; SCLEROTIC SKIN; GROWTH-FACTOR; ANIMAL-MODEL; TGF-BETA; cold atmospheric plasma; localized scleroderma; anti-fibrosis; bleomycin-induced fibrosis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Sep 2022 13:30 |
| Last Modified: | 06 Sep 2022 13:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46730 |
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