Noda, Kentaro and Dufner, Bianca and Ito, Haruyasu and Yoshida, Ken and Balboni, Gianfranco and Straub, Rainer H. (2021) Differential inflammation-mediated function of prokineticin 2 in the synovial fibroblasts of patients with rheumatoid arthritis compared with osteoarthritis. SCIENTIFIC REPORTS, 11 (1): 18399. ISSN 2045-2322,
Full text not available from this repository. (Request a copy)Abstract
Prokineticin 2 (PK2) is a secreted protein involved in several pathological and physiological processes, including the regulation of inflammation, sickness behaviors, and circadian rhythms. Recently, it was reported that PK2 is associated with the pathogenesis of collagen-induced arthritis in mice. However, the role of PK2 in the pathogenesis of rheumatoid arthritis (RA) or osteoarthritis (OA) remains unknown. In this study, we collected synovial tissue, plasma, synovial fluid, and synovial fibroblasts (SF) from RA and OA patients to analyze the function of PK2 using immunohistochemistry, enzyme-linked immunosorbent assays, and tissue superfusion studies. PK2 and its receptors prokineticin receptor (PKR) 1 and 2 were expressed in RA and OA synovial tissues. PKR1 expression was downregulated in RA synovial tissue compared with OA synovial tissue. The PK2 concentration was higher in RA synovial fluid than in OA synovial fluid but similar between RA and OA plasma. PK2 suppressed the production of IL-6 from TNF alpha-prestimulated OA-SF, and this effect was attenuated in TNF alpha-prestimulated RA-SF. This phenomenon was accompanied by the upregulation of PKR1 in OA-SF. This study provides a new model to explain some aspects underlying the chronicity of inflammation in RA.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ACTIVATED PROTEIN-KINASE; MAMMALIAN PROKINETICINS; AMPHIBIAN HOMOLOG; NERVOUS-SYSTEM; BV8; CELLS; IDENTIFICATION; INVOLVEMENT; SUPPRESSION; CONVERSION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Sep 2022 13:20 |
| Last Modified: | 09 Sep 2022 13:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/46951 |
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