Chanyalew, Menberework and Abebe, Markos and Endale, Birtukan and Girma, Selfu and Tasew, Geremew and van Zandbergen, Ger and Ritter, Uwe and Gadisa, Endalamaw and Aseffa, Abraham and Laskay, Tamas (2021) Enhanced production of pro-inflammatory cytokines and chemokines in Ethiopian cutaneous leishmaniasis upon exposure to Leishmania aethiopica. CYTOKINE, 145: 155289. ISSN 1043-4666, 1096-0023
Full text not available from this repository. (Request a copy)Abstract
The clinical course and outcome of cutaneous leishmaniasis (CL) vary due to the infecting Leishmania species and host genetic makeup that result in different immune responses against the parasites. The host immune response to Leishmania aethiopica (L. aethiopica), the causative agent of CL in Ethiopia, is poorly understood. To contribute to the understanding of the protective immune response in CL due to L. aethiopica, we characterized the cytokine response to L. aethiopica in patients with the localized form of CL (LCL) and age-and sex-matched apparently healthy controls. By applying a whole blood based in vitro culture we found enhanced release of TNF, IL-6, MCP1 or CCL2, IP-10 or CXCL10, MIP-113 or CCL4 and IL-8 or CXCL8- but not of IL-10CL patients in response to L. aethiopica compared to the controls. No difference was observed between LCL cases and controls in the secretion of these cytokines and chemokines in whole blood cultures treated with the TLR-ligands LPS, MALP-2 or poly I: C. The observed increased secretion of the pro-inflammatory cytokines/chemokines reflects an enhanced response against the parasites by LCL patients as compared to healthy controls rather than a generally enhanced ability of blood leukocytes from LCL patients to respond to microbial constituents. Our findings suggest that the enhanced production of pro-inflammatory cytokines/chemokines is associated with localized cutaneous leishmaniasis caused by L. aethiopica.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MONOCYTE CHEMOTACTIC PROTEIN-1; IMMUNE-RESPONSES; INTERFERON-GAMMA; IFN-GAMMA; INFECTION; SUSCEPTIBILITY; RESISTANCE; IL-1-BETA; MICE; PROMASTIGOTES; Cutaneous leishmaniasis; Leishmania aethiopica; Cytokine; Chemokine whole blood assay |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2022 04:13 |
| Last Modified: | 12 Sep 2022 04:13 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47015 |
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