Nogueira, Andressa Vilas Boas and Nokhbehsaim, Marjan and Damanaki, Anna and Eick, Sigrun and Kirschneck, Christian and Schroeder, Agnes and Jantsch, Jonathan and Deschner, James (2021) Filifactor alocis and Tumor Necrosis Factor-Alpha Stimulate Synthesis of Visfatin by Human Macrophages. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22 (3): 1235. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
There is little known about the effect of the periodontopathogen Filifactor alocis on macrophages as key cells of the innate immune defense in the periodontium. Therefore, the aim of the present study was to investigate the effect of F. alocis and additionally of the pro-inflammatory cytokine tumor necrosis factor-alpha (TNF alpha) on visfatin and other pro-inflammatory and proteolytic molecules associated with periodontitis in human macrophages. The presence of macrophage markers CD14, CD86, CD68, and CD163 was examined in gingival biopsies from healthy individuals and periodontitis patients. Human macrophages were incubated with F. alocis and TNF alpha for up to 2 d. The effects of both stimulants on macrophages were determined by real-time PCR, ELISA, immunocytochemistry, and immunofluorescence. F. alocis was able to significantly stimulate the synthesis of visfatin by human macrophages using TLR2 and MAPK pathways. In addition to visfatin, F. alocis was also able to increase the synthesis of cyclooxygenase 2, TNF alpha, and matrix metalloproteinase 1. Like F. alocis, TNF alpha was also able to stimulate the production of these proinflammatory and proteolytic molecules. Our results highlight the pathogenetic role of F. alocis in periodontal diseases and also underline the involvement of visfatin in the aetiopathogenesis of periodontitis.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; periodontitis; Filifactor alocis; tumor necrosis factor; visfatin; macrophage; COX2; MMP1 |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Kieferorthopädie Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2022 04:16 |
| Last Modified: | 12 Sep 2022 04:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47017 |
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