Wagner-Drouet, Eva and Teschner, Daniel and Wolschke, Christine and Schaefer-Eckart, Kerstin and Gaertner, Johannes and Mielke, Stephan and Schreder, Martin and Kobbe, Guido and Hilgendorf, Inken and Klein, Stefan and Verbeek, Mareike and Ditschkowski, Markus and Koch, Martina and Lindemann, Monika and Schmidt, Traudel and Rascle, Anne and Barabas, Sascha and Deml, Ludwig and Wagner, Ralf and Wolff, Daniel (2021) Comparison of Cytomegalovirus-Specific Immune Cell Response to Proteins versus Peptides Using an IFN-gamma ELISpot Assay after Hematopoietic Stem Cell Transplantation. DIAGNOSTICS, 11 (2): 312. ISSN , 2075-4418
Full text not available from this repository. (Request a copy)Abstract
Cytomegalovirus (CMV) infection is a major cause of morbidity and mortality following hematopoietic stem cell transplantation (HSCT). Measuring CMV-specific cellular immunity may improve the risk stratification and management of patients. IFN-gamma ELISpot assays, based on the stimulation of peripheral blood mononuclear cells with CMV pp65 and IE-1 proteins or peptides, have been validated in clinical settings. However, it remains unclear to which extend the T-cell response to synthetic peptides reflect that mediated by full-length proteins processed by antigen-presenting cells. We compared the stimulating ability of pp65 and IE-1 proteins and corresponding overlapping peptides in 16 HSCT recipients using a standardized IFN-gamma ELISpot assay. Paired qualitative test results showed an overall 74.4% concordance. Discordant results were mainly due to low-response tests, with one exception. One patient with early CMV reactivation and graft-versus-host disease, sustained CMV DNAemia and high CD8(+) counts showed successive negative protein-based ELISpot results but a high and sustained response to IE-1 peptides. Our results suggest that the response to exogenous proteins, which involves their uptake and processing by antigen-presenting cells, more closely reflects the physiological response to CMV infection, while the response to exogenous peptides may lead to artificial in vitro T-cell responses, especially in strongly immunosuppressed patients.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; CMV; CMV-specific cellular immunity; hematopoietic stem cell transplantation; recall antigen; peptide; immune monitoring; IFN-gamma ELISpot; T cells; antigen processing and presentation; immunosuppression |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2022 07:41 |
| Last Modified: | 12 Sep 2022 07:41 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47171 |
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