Voegel, Clarissa Daniela and Baumgartner, Markus R. and Kraemer, Thomas and Wuest, Stefan and Binz, Tina Maria (2021) Simultaneous quantification of steroid hormones and endocannabinoids (ECs) in human hair using an automated supported liquid extraction (SLE) and LC-MS/MS - Insights into EC baseline values and correlation to steroid concentrations. TALANTA, 222: 121499. ISSN 0039-9140, 1873-3573
Full text not available from this repository. (Request a copy)Abstract
Endogenous steroid hormones and endocannabinoids (ECs) are important regulators in the stress response of the human body. For the measurement of chronic stress, hair analysis has been established as method of choice for long-term and retrospective determination of endogenous stress markers. A sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the quantification of five steroid hormones (cortisone, cortisol, androstenedione, testosterone, progesterone) and four endocannabinoids (anandamide, palmitoylethanolamide, 2-arachidonylglycerol, oleoylethanolamide) in hair was developed and validated. The hair samples were extracted with methanol and cleaned up with a fully automated supported liquid extraction (SLE) before analysis. Special attention was paid to the difficulties accompanying the quantification of endogenous analytes in hair. Five different strategies for endogenous compound quantification in hair (surrogate analyte, standard addition, background correction, stripped matrix and solvent calibration) were tested and compared. As a result, the approach of the surrogate analyte was used for the quantification of steroid hormones whereas background correction was used for endocannabinoids. The measurement of 58 samples from healthy young adults allowed insights into endocannabinoid ranges in hair and the correlation to steroid hormones. No significant differences in steroid and EC concentration levels of male and female in hair were found, except for testosterone (p < 0.001) and androstenedione (p < 0.0001). Cortisol to cortisone and testosterone to androstenedione concentrations were significantly and positively correlated. There were significant intercorrelations between endocannabinoids.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | QUANTITATIVE-ANALYSIS; ENDOGENOUS CORTISOL; FEEDBACK INHIBITION; HUMAN PLASMA; VALIDATION; GLUCOCORTICOIDS; CHROMATOGRAPHY; DETERMINANTS; REDUCTIONS; SUBSTANCES; Liquid chromatography-tandem mass spectrometry; Steroid hormones; Endocannabinoids; Hair matrix; Endogenous substance quantification |
| Subjects: | 100 Philosophy & psychology > 150 Psychology 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Human Sciences > Institut für Psychologie > Lehrstuhl für Psychologie VII (Medizinische Psychologie, Psychologische Diagnostik und Methodenlehre) - Prof. Dr. Brigitte Kudielka |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Sep 2022 06:06 |
| Last Modified: | 06 Sep 2022 06:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47203 |
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