Taeger, Christian D. and Friedrich, Oliver and Horch, Raymund E. and Drechsler, Caroline and Weigand, Annika and Hopf, Fabio and Geppert, Carol and Muench, Frank and Birkholz, Torsten and Wenzel, Carina and Geis, Sebastian and Prantl, Lukas and Buchholz, Rainer and Praebst, Konstantin (2021) Extracorporeal perfusion - reduced to a one-way infusion. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION, 79 (2). pp. 257-267. ISSN 1386-0291, 1875-8622
Full text not available from this repository. (Request a copy)Abstract
BACKGROUND: Extracorporeal perfusion (EP) is moving into focus of research in reconstructive and transplantation medicine for the preservation of amputates and free tissue transplants. The idea behind EP is the reduction of ischemia-related cell damage between separation from blood circulation and reanastomosis of the transplant. Most experimental approaches are based on a complex system that moves the perfusate in a circular course. OBJECTIVE AND METHODS: In this study, we aimed to evaluate if a simple perfusion by an infusion bag filled with an electrolyte solution can provide acceptable results in terms of flow stability, oxygen supply and viability conservation for EP of a muscle transplant. The results are compared to muscles perfused with a pump system as well as muscles stored under ischemic conditions after a one-time intravasal flushing with Jonosteril. RESULTS: With this simple method a sufficient oxygen supply could be achieved and functionality could be maintained between 3.35 times and 4.60 times longer compared to the control group. Annexin V positive nuclei, indicating apoptosis, increased by 9.7% in the perfused group compared to 24.4% in the control group. CONCLUSIONS: Overall, by decreasing the complexity of the system, EP by one-way infusion can become more feasible in clinical situations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | SKELETAL-MUSCLE; AMPUTATED LIMBS; PRESERVATION; TISSUE; ISCHEMIA; SYSTEM; FLAPS; LUNG; Extracorporeal perfusion; external field stimulation; free flap perfusion; reperfusion; ischemia; free flap transplantation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Plastische-, Hand- und Wiederherstellungschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 12 Sep 2022 12:02 |
| Last Modified: | 12 Sep 2022 12:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47207 |
Actions (login required)
 |
View Item |
