Köfeler, Harald C. and Ahrends, Robert and Baker, Erin S. and Ekroos, Kim and Han, Xianlin and Hoffmann, Nils and Holcapek, Michal and Wenk, Markus R. and Liebisch, Gerhard (2021) Recommendations for good practice in MS-based lipidomics. JOURNAL OF LIPID RESEARCH, 62: 100138. ISSN 0022-2275, 1539-7262
Full text not available from this repository. (Request a copy)Abstract
In the last 2 decades, lipidomics has become one of the fastest expanding scientific disciplines in biomedical research. With an increasing number of new research groups to the field, it is even more important to design guidelines for assuring high standards of data quality. The Lipidomics Standards Initiative is a community-based endeavor for the coordination of development of these best practice guidelines in lipidomics and is embedded within the International Lipidomics Society. It is the intention of this review to highlight the most quality-relevant aspects of the lipidomics workflow, including preanalytics, sample preparation, MS, and lipid species identification and quantitation. Furthermore, this review just does not only highlights examples of best practice but also sheds light on strengths, drawbacks, and pitfalls in the lipidomic analysis workflow. While this review is neither designed to be a step-by-step protocol by itself nor dedicated to a specific application of lipidomics, it should nevertheless provide the interested reader with links and original publications to obtain a comprehensive overview concerning the state-of-the-art practices in the field.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | TANDEM MASS-SPECTROMETRY; ION MOBILITY SPECTROMETRY; COLLISIONAL-ACTIVATED DISSOCIATION; SOLID-PHASE EXTRACTION; SUPERCRITICAL-FLUID CHROMATOGRAPHY; HIGH-THROUGHPUT QUANTIFICATION; DRIVEN FRAGMENTATION PROCESSES; LIQUID-CHROMATOGRAPHY; SHOTGUN LIPIDOMICS; QUANTITATIVE-ANALYSIS; lipidomics; metabolomics; MS; chromatography; ion mobility spectrometry; phospholipids; sphingolipids; LC-MS; lipid identification |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin |
| Depositing User: | Petra Gürster |
| Date Deposited: | 17 Jan 2023 08:40 |
| Last Modified: | 17 Jan 2023 08:40 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47263 |
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