Segues, Aina and van Duijnhoven, Sander M. J. and Parade, Marc and Driessen, Lilian and Vukovic, Natasa and Zaiss, Dietmar and Sijts, Alice J. A. M. and Berraondo, Pedro and van Elsas, Andrea (2021) Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies. JOURNAL OF IMMUNOLOGICAL METHODS, 499: 113173. ISSN 0022-1759, 1872-7905
Full text not available from this repository. (Request a copy)Abstract
Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNF alpha. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8(+) T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8(+) cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RECEPTOR; LYMPHOCYTES; EXPRESSION; INDUCTION; ALPHA; CD40; TNFR2; Antibody; Epitope; Cysteine-rich domain; Costimulation; Treg |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Sep 2022 08:17 |
| Last Modified: | 13 Sep 2022 08:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47277 |
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