Municio, Celia and Antosz, Wojciech and Grasser, Klaus D. and Kornobis, Etienne and Van Bel, Michiel and Eguinoa, Ignacio and Coppens, Frederik and Braeutigam, Andrea and Lermontova, Inna and Bruckmann, Astrid and Zelkowska, Katarzyna and Houben, Andreas and Schubert, Veit (2021) The Arabidopsis condensin CAP-D subunits arrange interphase chromatin. NEW PHYTOLOGIST, 230 (3). pp. 972-987. ISSN 0028-646X, 1469-8137
Full text not available from this repository. (Request a copy)Abstract
Condensins are best known for their role in shaping chromosomes. Other functions such as organizing interphase chromatin and transcriptional control have been reported in yeasts and animals, but little is known about their function in plants. To elucidate the specific composition of condensin complexes and the expression of CAP-D2 (condensin I) and CAP-D3 (condensin II), we performed biochemical analyses in Arabidopsis. The role of CAP-D3 in interphase chromatin organization and function was evaluated using cytogenetic and transcriptome analysis in cap-d3 T-DNA insertion mutants. CAP-D2 and CAP-D3 are highly expressed in mitotically active tissues. In silico and pull-down experiments indicate that both CAP-D proteins interact with the other condensin I and II subunits. In cap-d3 mutants, an association of heterochromatic sequences occurs, but the nuclear size and the general histone and DNA methylation patterns remain unchanged. Also, CAP-D3 influences the expression of genes affecting the response to water, chemicals, and stress. The expression and composition of the condensin complexes in Arabidopsis are similar to those in other higher eukaryotes. We propose a model for the CAP-D3 function during interphase in which CAP-D3 localizes in euchromatin loops to stiffen them and consequently separates centromeric regions and 45S rDNA repeats.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; Arabidopsis thaliana; chromatin organization; chromosomes; condensin; interphase nuclei; SMC proteins |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie (Prof. Dr. Klaus Grasser) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Sep 2022 12:50 |
| Last Modified: | 13 Sep 2022 12:50 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47325 |
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