Korkmaz, Yueksel and Puladi, Behrus and Galler, Kerstin and Kaemmerer, Peer W. and Schroeder, Agnes and Goelz, Lina and Sparwasser, Tim and Bloch, Wilhelm and Friebe, Andreas and Deschner, James (2021) Inflammation in the Human Periodontium Induces Downregulation of the alpha(1)- and beta(1)-Subunits of the sGC in Cementoclasts. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 22 (2): 539. ISSN , 1422-0067
Full text not available from this repository. (Request a copy)Abstract
Nitric oxide (NO) binds to soluble guanylyl cyclase (sGC), activates it in a reduced oxidized heme iron state, and generates cyclic Guanosine Monophosphate (cGMP), which results in vasodilatation and inhibition of osteoclast activity. In inflammation, sGC is oxidized and becomes insensitive to NO. NO- and heme-independent activation of sGC requires protein expression of the alpha(1)- and beta(1)-subunits. Inflammation of the periodontium induces the resorption of cementum by cementoclasts and the resorption of the alveolar bone by osteoclasts, which can lead to tooth loss. As the presence of sGC in cementoclasts is unknown, we investigated the alpha(1)- and beta(1)-subunits of sGC in cementoclasts of healthy and inflamed human periodontium using double immunostaining for CD68 and cathepsin K and compared the findings with those of osteoclasts from the same sections. In comparison to cementoclasts in the healthy periodontium, cementoclasts under inflammatory conditions showed a decreased staining intensity for both alpha(1)- and beta(1)-subunits of sGC, indicating reduced protein expression of these subunits. Therefore, pharmacological activation of sGC in inflamed periodontal tissues in an NO- and heme-independent manner could be considered as a new treatment strategy to inhibit cementum resorption.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; nitric oxide; soluble guanylyl cyclase; cGMP; cementoclasts; cementum; osteoclasts; alveolar bone; periodontitis |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Sep 2022 12:16 |
| Last Modified: | 14 Sep 2022 12:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47478 |
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