Chernomor, Olga and Peters, Lena and Schneidewind, Judith and Loeschcke, Anita and Knieps-Gruenhagen, Esther and Schmitz, Fabian and von Lieres, Eric and Kutta, Roger Jan and Svensson, Vera and Jaeger, Karl-Erich and Drepper, Thomas and von Haeseler, Arndt and Krauss, Ulrich (2021) Complex Evolution of Light-Dependent Protochloronllyllide Oxidoreductases in Aerobic Anoxygenic Phototrophs: origin, Phylogeny, and Function. MOLECULAR BIOLOGY AND EVOLUTION, 38 (3). pp. 819-837. ISSN 0737-4038, 1537-1719
Full text not available from this repository. (Request a copy)Abstract
Light-dependent protochlorophyllide oxidoreductase (LPOR) and dark-operative protochlorophyllide oxidoreductase are evolutionary and structurally distinct enzymes that are essential for the synthesis of (bacterio)chlorophyll, the primary pigment needed for both anoxygenic and oxygenic photosynthesis. In contrast to the long-held hypothesis that LPORs are only present in oxygenic phototrophs, we recently identified a functional LPOR in the aerobic anoxygenic phototrophic bacterium (AAPB) Dinoroseobacter shibae and attributed its presence to a single horizontal gene transfer event from cyanobacteria. Here, we provide evidence for the more widespread presence of genuine LPOR enzymes in AAPBs. An exhaustive bioinformatics search identified 36 putative LPORs outside of oxygenic phototrophic bacteria (cyanobacteria) with the majority being AAPBs. Using in vitro and in vivo assays, we show that the large majority of the tested AAPB enzymes are genuine LPORs. Solution structural analyses, performed for two of the AAPB LPORs, revealed a globally conserved structure when compared with a well-characterized cyanobacterial LPOR. Phylogenetic analyses suggest that LPORs were transferred not only from cyanobacteria but also subsequently between proteobacteria and from proteobacteria to Gemmatimonadetes. Our study thus provides another interesting example for the complex evolutionary processes that govern the evolution of bacteria, involving multiple horizontal gene transfer events that likely occurred at different time points and involved different donors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NADPH-PROTOCHLOROPHYLLIDE OXIDOREDUCTASE; HORIZONTAL GENE-TRANSFER; SP-NOV.; CHLOROPHYLL BIOSYNTHESIS; BACTERIOCHLOROPHYLL-A; BIOLOGICAL MACROMOLECULES; KINETIC CHARACTERIZATION; RHODOBACTER-CAPSULATUS; SOLUTION SCATTERING; STRUCTURAL BIOLOGY; light-driven enzyme; chlorophyll biosynthesis; evolution; aerobic anoxygenic phototrophic bacteria; photosynthesis |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Physical Chemistry I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 08 Sep 2022 06:11 |
| Last Modified: | 08 Sep 2022 06:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47516 |
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