Del Rosario, Joanne Marie M. and da Costa, Kelly A. S. and Asbach, Benedikt and Ferrara, Francesca and Ferrari, Matteo and Wells, David A. and Mann, Gurdip Singh and Ameh, Veronica O. and Sabeta, Claude T. and Banyard, Ashley C. and Kinsley, Rebecca and Scott, Simon D. and Wagner, Ralf and Heeney, Jonathan L. and Carnell, George W. and Temperton, Nigel J. (2021) Exploiting Pan Influenza A and Pan Influenza B Pseudotype Libraries for Efficient Vaccine Antigen Selection. VACCINES, 9 (7): 741. ISSN , 2076-393X
Full text not available from this repository. (Request a copy)Abstract
We developed an influenza hemagglutinin (HA) pseudotype library encompassing Influenza A subtypes HA1-18 and Influenza B subtypes (both lineages) to be employed in influenza pseudotype microneutralization (pMN) assays. The pMN is highly sensitive and specific for detecting virus-specific neutralizing antibodies against influenza viruses and can be used to assess antibody functionality in vitro. Here we show the production of these viral HA pseudotypes and their employment as substitutes for wildtype viruses in influenza neutralization assays. We demonstrate their utility in detecting serum responses to vaccination with the ability to evaluate cross-subtype neutralizing responses elicited by specific vaccinating antigens. Our findings may inform further preclinical studies involving immunization dosing regimens in mice and may help in the creation and selection of better antigens for vaccine design. These HA pseudotypes can be harnessed to meet strategic objectives that contribute to the strengthening of global influenza surveillance, expansion of seasonal influenza prevention and control policies, and strengthening pandemic preparedness and response.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HEMAGGLUTININ MEMBRANE GLYCOPROTEIN; PATHOGENIC AVIAN INFLUENZA; RECEPTOR-BINDING; GLOBAL MORTALITY; GENE DELIVERY; VIRUS; ANTIBODY; NEUTRALIZATION; H5N1; EVOLUTION; influenza; hemagglutinin; pseudotype; vaccine; immunogenicity; monoclonal anti-body; neutralization |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2022 15:34 |
| Last Modified: | 16 Sep 2022 15:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47554 |
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