Meyer, Stefanie and Handke, Diana and Mueller, Anja and Biehl, Katharina and Kreuz, Markus and Bukur, Juergen and Koehl, Ulrike and Lazaridou, Maria-Filothei and Berneburg, Mark and Steven, Andre and Massa, Chiara and Seliger, Barbara (2021) Distinct Molecular Mechanisms of Altered HLA Class II Expression in Malignant Melanoma. CANCERS, 13 (15): 3907. ISSN , 2072-6694
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Simple Summary There exists limited knowledge about the underlying molecular processes controlling the expression of HLA class II APM components and their prognostic significance in melanoma. Therefore, this study analyzed the basal and regulated expression of HLA class II antigens and components in melanoma cell lines and patients' lesions in conjunction to T-cell infiltration. The heterogeneous constitutive HLA class II APM expression was caused by distinct molecular mechanisms and was partially linked to immune cell infiltration and clinical parameters. These results contribute not only to a better understanding of the regulation of HLA class II expression in melanoma, but might have an impact on the design of novel (immuno)therapies for the treatment of this disease. Background: The human leukocyte antigen (HLA) class II molecules are constitutively expressed in some melanoma, but the underlying molecular mechanisms have not yet been characterized. Methods: The expression of HLA class II antigen processing machinery (APM) components was determined in melanoma samples by qPCR, Western blot, flow cytometry and immunohistochemistry. Immunohistochemical and TCGA datasets were used for correlation of HLA class II expression to tumor grading, T-cell infiltration and patients' survival. Results: The heterogeneous HLA class II expression in melanoma samples allowed us to characterize four distinct phenotypes. Phenotype I totally lacks constitutive HLA class II surface expression, which is inducible by interferon-gamma (IFN-gamma); phenotype II expresses low basal surface HLA class II that is further upregulated by IFN-gamma; phenotype III lacks constitutive and IFN-gamma controlled HLA class II expression, but could be induced by epigenetic drugs; and in phenotype IV, lack of HLA class II expression is not recovered by any drug tested. High levels of HLA class II APM component expression were associated with an increased intra-tumoral CD4+ T-cell density and increased patients' survival. Conclusions: The heterogeneous basal expression of HLA class II antigens and/or APM components in melanoma cells is caused by distinct molecular mechanisms and has clinical relevance.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MHC CLASS-II; CIITA PROMOTER-IV; CD4(+) T-CELLS; ANTIGEN-EXPRESSION; IFN-GAMMA; TRANSCRIPTIONAL REGULATION; DIFFERENTIAL EXPRESSION; TRANSACTIVATOR CIITA; HISTONE ACETYLATION; INTERFERON-GAMMA; HLA class II; CIITA; IFN; methylation; signal transduction |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 16 Sep 2022 15:35 |
| Last Modified: | 16 Sep 2022 15:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47557 |
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