Porizka, Pavel and Vytiskova, Karolina and Oborilova, Radka and Pastucha, Matej and Gabris, Ivo and Brandmeier, Julian C. and Modlitbova, Pavlina and Gorris, Hans H. and Novotny, Karel and Skladal, Petr and Kaiser, Jozef and Farka, Zdenek (2021) Laser-induced breakdown spectroscopy as a readout method for immunocytochemistry with upconversion nanoparticles. MICROCHIMICA ACTA, 188 (5): 147. ISSN 0026-3672, 1436-5073
Full text not available from this repository. (Request a copy)Abstract
Immunohistochemistry (IHC) and immunocytochemistry (ICC) are widely used to identify cancerous cells within tissues and cell cultures. Even though the optical microscopy evaluation is considered the gold standard, the limited range of useful labels and narrow multiplexing capabilities create an imminent need for alternative readout techniques. Laser-induced breakdown spectroscopy (LIBS) enables large-scale multi-elemental analysis of the surface of biological samples, e.g., thin section or cell pellet. It is, therefore, a potential alternative for IHC and ICC readout of various labels or tags (Tag-LIBS approach). Here, we introduce Tag-LIBS as a method for the specific determination of HER2 biomarker. The cell pellets were labeled with streptavidin-conjugated upconversion nanoparticles (UCNP) through a primary anti-HER2 antibody and a biotinylated secondary antibody. The LIBS scanning enabled detecting the characteristic elemental signature of yttrium as a principal constituent of UCNP, thus indirectly providing a reliable way to differentiate between HER2-positive BT-474 cells and HER2-negative MDA-MB-231 cells. The comparison of results with upconversion optical microscopy and luminescence intensity scanning confirmed that LIBS is a promising alternative for the IHC and ICC readout.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ; Immunocytochemistry; Immunohistochemistry; Laser-induced breakdown spectroscopy; Tag-LIBS; Photon-upconversion nanoparticles |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Sep 2022 06:35 |
| Last Modified: | 20 Sep 2022 06:35 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47595 |
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