Gallerand, Alexandre and Stunault, Marion and Merlin, Johanna and Luehmann, Hannah P. and Sultan, Deborah H. and Firulyova, Maria M. and Magnone, Virginie and Khedher, Narges and Jalil, Antoine and Dolfi, Bastien and Castiglione, Alexia and Dumont, Adelie and Ayrault, Marion and Vaillant, Nathalie and Gilleron, Jerome and Barbry, Pascal and Dombrowicz, David and Mack, Matthias and Masson, David and Bertero, Thomas and Becher, Burkhard and Williams, Jesse W. and Zaitsev, Konstantin and Liu, Yongjian and Guinamard, Rodolphe R. and Yvan-Charvet, Laurent and Ivanov, Stoyan (2021) Brown adipose tissue monocytes support tissue expansion. NATURE COMMUNICATIONS, 12 (1): 5255. ISSN 2041-1723
Full text not available from this repository. (Request a copy)Abstract
Monocytes are part of the mononuclear phagocytic system. Monocytes play a central role during inflammatory conditions and a better understanding of their dynamics might open therapeutic opportunities. In the present study, we focused on the characterization and impact of monocytes on brown adipose tissue (BAT) functions during tissue remodeling. Single-cell RNA sequencing analysis of BAT immune cells uncovered a large diversity in monocyte and macrophage populations. Fate-mapping experiments demonstrated that the BAT macrophage pool requires constant replenishment from monocytes. Using a genetic model of BAT expansion, we found that brown fat monocyte numbers were selectively increased in this scenario. This observation was confirmed using a CCR2-binding radiotracer and positron emission tomography. Importantly, in line with their tissue recruitment, blood monocyte counts were decreased while bone marrow hematopoiesis was not affected. Monocyte depletion prevented brown adipose tissue expansion and altered its architecture. Podoplanin engagement is strictly required for BAT expansion. Together, these data redefine the diversity of immune cells in the BAT and emphasize the role of monocyte recruitment for tissue remodeling. Adipose tissue is composed of a number of adipocytes and a number of other cells including immune cells. Here the authors use single-cell sequencing of murine brown adipose tissue immune cells and describe multiple macrophage and monocyte subsets and show that monocytes contribute to brown adipose tissue expansion.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COLD-INDUCED THERMOGENESIS; DENDRITIC CELLS; BONE-MARROW; MACROPHAGES; MOBILIZATION; ORCHESTRATE; EXPRESSION; LIPOLYSIS; DIFFERENTIATION; RECRUITMENT |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Nephrologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Sep 2022 06:02 |
| Last Modified: | 14 Sep 2022 06:02 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47692 |
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