Henkel, Jan and Savi, Flavia Medeiros and Berner, Arne and Fountain, Stephanie and Saifzadeh, Siamak and Steck, Roland and Epari, Devakar R. and Woodruff, Maria A. and Knackstedt, Mark and Schuetz, Michael A. and Hutmacher, Dietmar W. (2021) Scaffold-guided bone regeneration in large volume tibial segmental defects. BONE, 153: 116163. ISSN 8756-3282, 1873-2763
Full text not available from this repository. (Request a copy)Abstract
Large volume losses in weight bearing long bones are a major challenge in clinical practice. Despite multiple innovations over the last decades, significant limitations subsist in current clinical treatment options which is driving a strong clinical demand for clinically translatable treatment alternatives, including bone tissue engineering applications. Despite these shortcomings, preclinical large animal models of large volume segmental bone defects to investigate the regenerative capacity of bone tissue engineering strategies under clinically relevant conditions are rarely described in literature. We herein present a newly established preclinical ovine animal model for the treatment of XL volume (19 cm(3)) segmental tibial defects. In eight aged male Merino sheep (age > 6 years) a mid-diaphyseal tibial segmental defect was created and stabilized with a 5.6 mm Dynamic Compression Plate (DCP). We present short-term (3 months) and long-term (12-15 months) results of a pilot study using medical grade Polycaprolactone-Tricalciumphosphate (mPCL-TCP) scaffolds combined with a dose of 2 mg rhBMP-7 delivered in Platelet-Rich- Plasma (PRP). Furthermore, detailed analyses of the mechanical properties of the scaffolds as well as interfragmentary movement (IFM) and DCP-surface strain in vitro and a comprehensive description of the surgical and post-surgery protocol and post-mortem analysis is given.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MORPHOGENETIC PROTEINS; MECHANICAL-PROPERTIES; PRECLINICAL MODELS; GROWTH-FACTORS; STEM-CELLS; TISSUE; REPAIR; RECONSTRUCTION; IMPLANTATION; ARCHITECTURE; Large volume bone defect; Bone tissue engineering; Bone morphogenetic protein; Polycaprolactone; Scaffold; Sheep; Ovine; Preclinical animal model |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 20 Sep 2022 14:30 |
| Last Modified: | 20 Sep 2022 14:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47729 |
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