Steenblock, Charlotte and Richter, Stefanie and Berger, Ilona and Barovic, Marko and Schmid, Janine and Schubert, Undine and Jarzebska, Natalia and von Maessenhausen, Anne and Linkermann, Andreas and Schuermann, Annette and Pablik, Jessica and Dienemann, Thomas and Evert, Katja and Rodionov, Roman N. and Semenova, Natalia Y. and Zinserling, Vsevolod A. and Gainetdinov, Raul R. and Baretton, Gustavo and Lindemann, Dirk and Solimena, Michele and Ludwig, Barbara and Bornstein, Stefan R. (2021) Viral infiltration of pancreatic islets in patients with COVID-19. NATURE COMMUNICATIONS, 12 (1): 3534. ISSN 2041-1723,
Full text not available from this repository. (Request a copy)Abstract
Metabolic diseases are associated with an increased risk of severe COVID-19 and conversely, new-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients. Here, we performed a comprehensive analysis of pancreatic autopsy tissue from COVID-19 patients using immunofluorescence, immunohistochemistry, RNA scope and electron microscopy and detected SARS-CoV-2 viral infiltration of beta-cells in all patients. Using SARS-CoV-2 pseudoviruses, we confirmed that isolated human islet cells are permissive to infection. In eleven COVID-19 patients, we examined the expression of ACE2, TMPRSS and other receptors and factors, such as DPP4, HMBG1 and NRP1, that might facilitate virus entry. Whereas 70% of the COVID-19 patients expressed ACE2 in the vasculature, only 30% displayed ACE2-expression in beta-cells. Even in the absence of manifest new-onset diabetes, necroptotic cell death, immune cell infiltration and SARS-CoV-2 viral infection of pancreatic beta-cells may contribute to varying degrees of metabolic dysregulation in patients with COVID-19. New-onset hyperglycemia and complications of preexisting diabetes have been observed in COVID-19 patients, however, the underlying mechanisms are not fully understood. Here, the authors show that SARS-CoV-2 is detectable in both endocrine and exocrine cells of the pancreata of patients with COVID-19.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ENTRY FACTORS; ACE2; NECROPTOSIS; DOWNSTREAM; EXPRESSION; TMPRSS2; SYSTEM; MLKL; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Pathologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 21 Sep 2022 07:56 |
| Last Modified: | 21 Sep 2022 07:56 |
| URI: | https://pred.uni-regensburg.de/id/eprint/47781 |
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