Genewsky, Andreas and Jost, Ingmar and Busch, Catharina and Huber, Christian and Stindl, Julia and Skerka, Christine and Zipfel, Peter F. and Rohrer, Baerbel and Strauss, Olaf (2015) Activation of endogenously expressed ion channels by active complement in the retinal pigment epithelium. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 467 (10). pp. 2179-2191. ISSN 0031-6768, 1432-2013
Full text not available from this repository. (Request a copy)Abstract
Defective regulation of the alternative pathway of the complement system is believed to contribute to damage of retinal pigment epithelial (RPE) cells in age-related macular degeneration. Thus we investigated the effect of complement activation on the RPE cell membrane by analyzing changes in membrane conductance via patch-clamp techniques and Ca2+ imaging. Exposure of human ARPE-19 cells to complement-sufficient normal human serum (NHS) (25 %) resulted in a biphasic increase in intracellular free Ca2+ ([Ca2+](i)); an initial peak followed by sustained Ca2+ increase. C5- or C7-depleted sera did not fully reproduce the signal generated by NHS. The initial peak of the Ca2+ response was reduced by sarcoplasmic Ca2+-ATPase inhibitor thapsigargin, L-type channel blockers (R)-(+)-BayK8644 and isradipine, transient-receptor-potential (TRP) channel blocker ruthenium-red and ryanodine receptor blocker dantrolene. The sustained phase was carried by Ca(V)1.3 L-type channels via tyrosine-phosphorylation. Changes in [Ca2+](I) were accompanied by an abrupt hyperpolarization, resulting from a transient increase in membrane conductance, which was absent under extracellular Ca2+- or K+-free conditions and blocked by (R)-(+)-BayK8644 or paxilline, a maxiK channel inhibitor. Single-channel recordings confirmed the contribution of maxiK channels. Primary porcine RPE cells responded to NHS in a comparable manner. Pre-incubation with NHS reduced H2O2-induced cell death. In summary, in a concerted manner, C3a, C5a and sC5b-9 increased [Ca2+](i) by ryanodine-receptor-dependent activation of L-type channels in addition to maxi-K channels and TRP channels absent from any insertion of a lytic pore.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MEMBRANE-ATTACK-COMPLEX; HUMAN POLYMORPHONUCLEAR LEUKOCYTES; INTRACELLULAR FREE CALCIUM; MACULAR DEGENERATION; VEGF SECRETION; CA2+ CHANNELS; FACTOR-H; CELLS; FIBROBLASTS; INCREASE; (4-6): complement system; Retinal pigment epithelium; RPE; L-type channels; BK channels |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Augenheilkunde |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jun 2019 07:03 |
| Last Modified: | 07 Jun 2019 07:03 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4799 |
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