Earls, Megan R. and Steinig, Eike J. and Monecke, Stefan and Castruita, Jose A. Samaniego and Simbeck, Alexandra and Schneider-Brachert, Wulf and Vremera, Teodora and Dorneanu, Olivia S. and Loncaric, Igor and Bes, Michele and Lacoma, Alicia and Aymerich, Cristina Prat and Wernery, Ulrich and Armengol-Porta, Marc and Blomfeldt, Anita and Duchene, Sebastian and Bartels, Mette D. and Ehricht, Ralf and Coleman, David C. (2021) Exploring the evolution and epidemiology of European CC1 MRSA-IV: tracking a multidrug- resistant community-associated meticillin-resistant Staphylococcus aureus clone. MICROBIAL GENOMICS, 7 (7): 000601. ISSN 2057-5858,
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This study investigated the evolution and epidemiology of the community-associated and multidrug-resistant Staphylococcus aureus clone European CC1-MRSA-IV. Whole-genome sequences were obtained for 194 European CC1-MRSA-IV isolates (189 of human and 5 of animal origin) from 12 countries, and 10 meticillin-susceptible precursors (from North-Eastern Romania; all of human origin) of the clone. Phylogenetic analysis was performed using a maximum-likelihood approach, a time-measured phylogeny was reconstructed using Bayesian analysis, and in silico microarray genotyping was performed to identify resistance, virulence-associated and SCCmec (staphylococcal cassette chromosome mec) genes. Isolates were typically sequence type 1 (190/204) and spa type t127 (183/204). Bayesian analysis indicated that European CC1-MRSA-IV emerged in approximately 1995 before undergoing rapid expansion in the late 1990s and 2000s, while spreading throughout Europe and into the Middle East. Phylogenetic analysis revealed an unstructured meticillin-resistant S. aureus (MRSA) population, lacking significant geographical or temporal clusters. The MRSA were genotypically multidrug-resistant, consistently encoded seh, and intermittently (34/194) encoded an undisrupted hlb gene with concomitant absence of the lysogenic phage-encoded genes sak and scn. All MRSA also harboured a characteristic similar to 5350 nt insertion in SCCmec adjacent to orfX. Detailed demographic data from Denmark showed that there, the clone is typically (25/35) found in the community, and often (10/35) among individuals with links to South-Eastern Europe. This study elucidated the evolution and epidemiology of European CC1-MRSA-IV, which emerged from a meticillin-susceptible lineage prevalent in North-Eastern Romania before disseminating rapidly throughout Europe.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | VIRULENCE; ALGORITHM; GENOME; CA-MRSA; European CC1-MRSA-IV clone; evolution; epidemiology; phylogenomics; transmission |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Sep 2022 09:28 |
| Last Modified: | 27 Sep 2022 09:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48053 |
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