Llamazares-Prada, Maria and Espinet, Elisa and Mijosek, Vedrana and Schwartz, Uwe and Lutsik, Pavlo and Tamas, Raluca and Richter, Mandy and Behrendt, Annika and Pohl, Stephanie T. and Benz, Naja P. and Muley, Thomas and Warth, Arne and Heussel, Claus Peter and Winter, Hauke and Landry, Jonathan J. M. and Herth, Felix J. F. and Mertens, Tinne C. J. and Karmouty-Quintana, Harry and Koch, Ina and Benes, Vladimir and Korbel, Jan O. and Waszak, Sebastian M. and Trumpp, Andreas and Wyatt, David M. and Stahl, Heiko F. and Plass, Christoph and Jurkowska, Renata Z. (2021) Versatile workflow for cell type-resolved transcriptional and epigenetic profiles from cryopreserved human lung. JCI INSIGHT, 6 (6): e140443. ISSN , 2379-3708
Full text not available from this repository. (Request a copy)Abstract
Complexity of lung microenvironment and changes in cellular composition during disease make it exceptionally hard to understand molecular mechanisms driving development of chronic lung diseases. Although recent advances in cell type-resolved approaches hold great promise for studying complex diseases, their implementation relies on local access to fresh tissue, as traditional tissue storage methods do not allow viable cell isolation. To overcome these hurdles, we developed a versatile workflow that allows storage of lung tissue with high viability, permits thorough sample quality check before cell isolation, and befits sequencing-based profiling. We demonstrate that cryopreservation enables isolation of multiple cell types from both healthy and diseased lungs. Basal cells from cryopreserved airways retain their differentiation ability, indicating that cellular identity is not altered by cryopreservation. Importantly, using RNA sequencing and EPIC Array, we show that gene expression and DNA methylation signatures are preserved upon cryopreservation, emphasizing the suitability of our workflow for omics profiling of lung cells. Moreover, we obtained high-quality single-cell RNA-sequencing data of cells from cryopreserved human lungs, demonstrating that cryopreservation empowers single-cell approaches. Overall, thanks to its simplicity, our workflow is well suited for prospective tissue collection by academic collaborators and biobanks, opening worldwide access to viable human tissue.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RNA-SEQ DATA; STEM-CELLS; II CELLS; PARENCHYMAL FIBROBLASTS; PRECISION MEDICINE; BASAL-CELLS; CANCER; AIRWAY; CULTURE; TISSUE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Sep 2022 12:10 |
| Last Modified: | 27 Sep 2022 12:10 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48071 |
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