Maleeva, Galyna and Nin-Hill, Alba and Rustler, Karin and Petukhova, Elena and Ponomareva, Daria and Mukhametova, Elvira and Gomila, Alexandre M. J. and Wutz, Daniel and Alfonso-Prieto, Mercedes and Koenig, Burkhard and Gorostiza, Pau and Bregestovski, Piotr (2021) Subunit-Specific Photocontrol of Glycine Receptors by Azobenzene-Nitrazepam Photoswitcher. ENEURO, 8 (1): 0294-20.20. ISSN , 2373-2822
Full text not available from this repository. (Request a copy)Abstract
Photopharmacology is a unique approach that through a combination of photochemistry methods and advanced life science techniques allows the study and control of specific biological processes, ranging from intracellular pathways to brain circuits. Recently, a first photochromic channel blocker of anion-selective GABA(A) receptors, the azobenzene-nitrazepam-based photochromic compound (Azo-NZ1), has been described. In the present study, using patch-clamp technique in heterologous system and in mice brain slices, site-directed mutagenesis and molecular modeling we provide evidence of the interaction of Azo-NZ1 with glycine receptors (GlyRs) and determine the molecular basis of this interaction. Glycinergic synaptic neurotransmission determines an important inhibitory drive in the vertebrate nervous system and plays a crucial role in the control of neuronal circuits in the spinal cord and brain stem. GlyRs are involved in locomotion, pain sensation, breathing, and auditory function, as well as in the development of such disorders as hyperekplexia, epilepsy, and autism. Here, we demonstrate that Azo-NZ1 blocks in a UV-dependent manner the activity of alpha 2 GlyRs (GlyR2), while being barely active on alpha 1 GlyRs (GlyR1). The site of Azo-NZ1 action is in the chloride-selective pore of GlyR at the 2' position of transmembrane helix 2 and amino acids forming this site determine the difference in Azo-NZ1 blocking activity between GlyR2 and GlyR1. This subunit-specific modulation is also shown on motoneurons of brainstem slices from neonatal mice that switch during development from expressing "fetal" GlyR2 to "adult" GlyR1 receptors.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEVELOPMENTAL-CHANGES; CRYSTAL-STRUCTURES; SYNAPTIC CURRENTS; OPTICAL SWITCHES; MESSENGER-RNAS; ALPHA-SUBUNIT; ION CHANNELS; RAT-BRAIN; BINDING; INHIBITION; brain slices; glycine receptors; hypoglossal motoneurons; molecular modelling; patch-clamp; photopharmacology |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 27 Sep 2022 13:17 |
| Last Modified: | 27 Sep 2022 13:17 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48095 |
Actions (login required)
 |
View Item |
