Hauptmann, Judith and Schraivogel, Daniel and Bruckmann, Astrid and Manickavel, Sudhir and Jakob, Leonhard and Eichner, Norbert and Pfaff, Janina and Urban, Marc and Sprunck, Stefanie and Hafner, Markus and Tuschl, Thomas and Deutzmann, Rainer and Meister, Gunter (2015) Biochemical isolation of Argonaute protein complexes by Ago-APP. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 112 (38). pp. 11841-11845. ISSN 0027-8424,
Full text not available from this repository. (Request a copy)Abstract
During microRNA (miRNA)-guided gene silencing, Argonaute (Ago) proteins interact with a member of the TNRC6/GW protein family. Here we used a short GW protein-derived peptide fused to GST and demonstrate that it binds to Ago proteins with high affinity. This allows for the simultaneous isolation of all Ago protein complexes expressed in diverse species to identify associated proteins, small RNAs, or target mRNAs. We refer to our method as "Ago protein Affinity Purification by Peptides" (Ago-APP). Furthermore, expression of this peptide competes for endogenous TNRC6 proteins, leading to global inhibition of miRNA function in mammalian cells.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MICRORNA TARGETS; GENE-EXPRESSION; RNA CLEAVAGE; BINDING; IDENTIFICATION; REPRESSION; RECOGNITION; ARABIDOPSIS; DISSECTION; FAMILY; Argonaute; small RNAs; microRNAs; GW proteins; RNAi |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Jun 2019 08:08 |
| Last Modified: | 07 Jun 2019 08:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/4815 |
Actions (login required)
 |
View Item |
