Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition)

Cossarizza, Andrea and Chang, Hyun-Dong and Radbruch, Andreas and Abrignani, Sergio and Addo, Richard and Akdis, Muebeccel and Andrae, Immanuel and Andreata, Francesco and Annunziato, Francesco and Arranz, Eduardo and Bacher, Petra and Bari, Sudipto and Barnaba, Vincenzo and Barros-Martins, Joana and Baumjohann, Dirk and Beccaria, Cristian G. and Bernardo, David and Boardman, Dominic A. and Borger, Jessica and Boettcher, Chotima and Brockmann, Leonie and Burns, Marie and Busch, Dirk H. and Cameron, Garth and Cammarata, Ilenia and Cassotta, Antonino and Chang, Yinshui and Chirdo, Fernando Gabriel and Christakou, Eleni and Cicin-Sain, Luka and Cook, Laura and Corbett, Alexandra J. and Cornelis, Rebecca and Cosmi, Lorenzo and Davey, Martin S. and De Biasi, Sara and De Simone, Gabriele and del Zotto, Genny and Delacher, Michael and Di Rosa, Francesca and Di Santo, James and Diefenbach, Andreas and Dong, Jun and Doerner, Thomas and Dress, Regine J. and Dutertre, Charles-Antoine and Eckle, Sidonia B. G. and Eede, Pascale and Evrard, Maximilien and Falk, Christine S. and Feuerer, Markus and Fillatreau, Simon and Fiz-Lopez, Aida and Follo, Marie and Foulds, Gemma A. and Froebel, Julia and Gagliani, Nicola and Galletti, Giovanni and Gangaev, Anastasia and Garbi, Natalio and Garrote, Jose Antonio and Geginat, Jens and Gherardin, Nicholas A. and Gibellini, Lara and Ginhoux, Florent and Godfrey, Dale I. and Gruarin, Paola and Haftmann, Claudia and Hansmann, Leo and Harpur, Christopher M. and Hayday, Adrian C. and Heine, Guido and Hernandez, Daniela Carolina and Herrmann, Martin and Hoelsken, Oliver and Huang, Qing and Huber, Samuel and Huber, Johanna E. and Huehn, Jochen and Hundemer, Michael and Hwang, William Y. K. and Iannacone, Matteo and Ivison, Sabine M. and Jaeck, Hans-Martin and Jani, Peter K. and Keller, Baerbel and Kessler, Nina and Ketelaars, Steven and Knop, Laura and Knopf, Jasmin and Koay, Hui-Fern and Kobow, Katja and Kriegsmann, Katharina and Kristyanto, H. and Krueger, Andreas and Kuehne, Jenny F. and Kunze-Schumacher, Heike and Kvistborg, Pia and Kwok, Immanuel and Latorre, Daniela and Lenz, Daniel and Levings, Megan K. and Lino, Andreia C. and Liotta, Francesco and Long, Heather M. and Lugli, Enrico and MacDonald, Katherine N. and Maggi, Laura and Maini, Mala K. and Mair, Florian and Manta, Calin and Manz, Rudolf Armin and Mashreghi, Mir-Farzin and Mazzoni, Alessio and McCluskey, James and Mei, Henrik E. and Melchers, Fritz and Melzer, Susanne and Mielenz, Dirk and Monin, Leticia and Moretta, Lorenzo and Multhoff, Gabriele and Munoz, Luis Enrique and Munoz-Ruiz, Miguel and Muscate, Franziska and Natalini, Ambra and Neumann, Katrin and Ng, Lai Guan and Niedobitek, Antonia and Niemz, Jana and Almeida, Larissa Nogueira and Notarbartolo, Samuele and Ostendorf, Lennard and Pallett, Laura J. and Patel, Amit A. and Percin, Gulce Itir and Peruzzi, Giovanna and Pinti, Marcello and Pockley, A. Graham and Pracht, Katharina and Prinz, Immo and Pujol-Autonell, Irma and Pulvirenti, Nadia and Quatrini, Linda and Quinn, Kylie M. and Radbruch, Helena and Rhys, Hefin and Rodrigo, Maria B. and Romagnani, Chiara and Saggau, Carina and Sakaguchi, Shimon and Sallusto, Federica and Sanderink, Lieke and Sandrock, Inga and Schauer, Christine and Scheffold, Alexander and Scherer, Hans U. and Schiemann, Matthias and Schildberg, Frank A. and Schober, Kilian and Schoen, Janina and Schuh, Wolfgang and Schueler, Thomas and Schulz, Axel R. and Schulz, Sebastian and Schulze, Julia and Simonetti, Sonia and Singh, Jeeshan and Sitnik, Katarzyna M. and Stark, Regina and Starossom, Sarah and Stehle, Christina and Szelinski, Franziska and Tan, Leonard and Tarnok, Attila and Tornack, Julia and Tree, Timothy I. M. and van Beek, Jasper J. P. and van de Veen, Willem and van Gisbergen, Klaas and Vasco, Chiara and Verheyden, Nikita A. and von Borstel, Anouk and Ward-Hartstonge, Kirsten A. and Warnatz, Klaus and Waskow, Claudia and Wiedemann, Annika and Wilharm, Anneke and Wing, James and Wirz, Oliver and Wittner, Jens and Yang, Jennie H. M. and Yang, Juhao (2021) Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition). EUROPEAN JOURNAL OF IMMUNOLOGY, 51 (12). pp. 2708-3145. ISSN 0014-2980, 1521-4141

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Abstract

The third edition of Flow Cytometry Guidelines provides the key aspects to consider when performing flow cytometry experiments and includes comprehensive sections describing phenotypes and functional assays of all major human and murine immune cell subsets. Notably, the Guidelines contain helpful tables highlighting phenotypes and key differences between human and murine cells. Another useful feature of this edition is the flow cytometry analysis of clinical samples and respective applications of flow cytometry in the context of a variety of autoimmune diseases, cancers as well as acute and chronic infectious diseases such as COVID-19. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid. All sections are written and peer-reviewed by leading flow cytometry experts and immunologists, making this edition an essential and state-of-the-art handbook for basic and clinical researchers.

Item Type: Article
Uncontrolled Keywords: REGULATORY T-CELLS; INNATE LYMPHOID-CELLS; HEMATOPOIETIC STEM-CELLS; MHC CLASS-I; NATURAL-KILLER-CELLS; ANTIGEN-SPECIFIC CD4(+); HUMAN BONE-MARROW; HUMAN B-CELL; ROR-GAMMA-T; HIGH-DIMENSIONAL CYTOMETRY;
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Immunologie
Medicine > Zentren des Universitätsklinikums Regensburg > Regensburger Centrum für Interventionelle Immunologie (RCI)
Depositing User: Dr. Gernot Deinzer
Date Deposited: 04 Oct 2022 10:23
Last Modified: 04 Oct 2022 10:23
URI: https://pred.uni-regensburg.de/id/eprint/48234

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