Lee, B. S. and Holler, Eggehard (1999) Effects of culture conditions on beta-poly(L-malate) production by Physarum polycephalum. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 51 (5). pp. 647-652. ISSN 0175-7598,
Full text not available from this repository.Abstract
Culture conditions for the fermentative production of beta-poly(L-malate) (PMLA) by microplasmodia of Physarum polycephalum were investigated and optimized. Optimal production was achieved in the presence of CaCO3. For 1.5% (w/v) D-glucose, 1% bactotryptone and 1% CaCO3, a maximum of 1.7 g PMLA/1 was secreted in 3 days. For 4.5% glucose and otherwise the same conditions, 2.7 g PMLA/1 was produced in 6 days. The contribution of carbonate was inhibited by avidin. PMLA and biomass production were not strictly coupled: PMLA was also synthesized at the beginning of the stationary phase. At pH 5.5 PMLA production was twice that at pH 4.0, while biomass was not changed. Optimal temperatures were 24-28 degrees C. (Fischer et al. 1989). Besides attracting interest as a physiological player (Angerer and Holler 1995; Schmidt et al. 1996), PMLA is pharmaceutically attractive for use in controlled drug release (Braud and Vert 1993). PMLA has been harvested from P. polycephalum plasmodia, routinely grown on the culture medium selected by Daniel and Baldwin (1964) for maximal biomass production. We now show that biomass and PMLA production can be dissociated, and a new culture formula is introduced for the purpose of optimal PMLA production.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DNA-POLYMERASE-ALPHA; AUREOBASIDIUM-PULLULANS; RHIZOPUS-ARRHIZUS; ACID) PRODUCTION; ACCUMULATION; PLASMODIA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Alumni or Retired > Prof. Dr. Eggehard Holler |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2022 12:09 |
| Last Modified: | 19 Oct 2022 12:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48255 |
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