Baeumler, W. and Abels, C. and Karrer, S. and Weiss, T. and Messmann, H. and Landthaler, Michael and Szeimies, Rolf-Markus (1999) Photo-oxidative killing of human colonic cancer cells using indocyanine green and infrared light. BRITISH JOURNAL OF CANCER, 80 (3-4). pp. 360-363. ISSN 0007-0920,
Full text not available from this repository. (Request a copy)Abstract
Despite of the approval of Photofrin(R) in various countries, chemically defined sensitizers for photodynamic therapy (PDT) are still needed for the absorption of light in the infrared spectrum, which provides a maximal penetration of light into tissue. Therefore, both the efficacy and the mechanism of action of the clinically approved dye indocyanine green (ICG) and laser irradiation were investigated in vitro. For the investigation of phototoxic effects, HT-29 cells were incubated 24 h prior to irradiation by using different concentrations of ICG (10-500 mu M). In each experiment, cells were irradiated using a continuous wave (cw)-diode laser (lambda(ex) = 805 nm, 30 J cm(-2), 40 mW cm(-2)). After laser irradiation, cell viability of dark control and of cells incubated with 500 mu M ICG was 1.27 +/- 0.11 or 0.28 +/- 0.05 respectively. Using 100 mu M ICG and D2O, cell viability was further decreased from 0.46 +/- 0.03 (H2O) to 0.11 +/- 0.01 (D2O). Using D2O and 100 mu M ICG, the concentration of malondialdehyde, a market of lipid peroxidation, increased from 0.89 +/- 0.10 nmol 10(-6) cells to 11.14 +/- 0.11 nmol 10(-6) cells. Using 100 mu M ICG and laser irradiation sodium azide or histidine (50 mM), quenchers of singlet oxygen reduced the cell killing significantly. In contrast, when using mannitol, a quencher of superoxide anion and hydroxyl radical, cell killing was not inhibited. According to the present results, photoactivated ICG seems to kill colonic cancer cells due to the generation of singlet oxygen and the subsequent formation of lipid peroxides. Therefore, ICG might present a promising photosensitizer for PDT; first clinical results confirm these findings.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PHOTODYNAMIC THERAPY; SINGLET OXYGEN; IN-VITRO; HEMATOPORPHYRIN; PHOTOSENSITIZERS; MICROSOMES; EFFICIENCY; MECHANISM; INVITRO; HT-29 cells; ICG; singlet oxygen; lipid peroxidation |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Innere Medizin I |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 19 Oct 2022 13:47 |
| Last Modified: | 19 Oct 2022 13:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/48264 |
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